Synthesis, electrospinning and in vitro test of a new biodegradable gelatin-based poly(ester urethane urea) for soft tissue engineering
Synthesis, electrospinning and in vitro test of a new biodegradable gelatin-based poly(ester urethane urea) for soft tissue engineering
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DOI：
10.1016/j.eurpolymj.2018.04.005
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发表时间：
2018-06
影响因子：
6
通讯作者：
T. Vieira;J. Silva;J. Borges;C. Henriques
中科院分区：
文献类型：
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作者：
T. Vieira;J. Silva;J. Borges;C. Henriques
生物可降解聚氨酯由于其可调节的物理化学性质而被研究作为组织工程支架材料。以聚己内酯二醇为软段，异佛尔酮二异氰酸酯和冷水鱼皮明胶为硬段，合成了一种可生物降解的明胶基聚氨酯脲。通过傅里叶变换红外光谱和质子核磁共振证实了合成，并通过热分析分析了在聚合物主链中引入明胶的量的影响。然后制备了明胶基聚氨酯脲静电纺丝纤维毡和溶剂流延膜，并对其物理化学和生物学性能进行了研究。它们呈现出疏水表面典型的无定形结构、弹性行为和水接触角。在磷酸盐缓冲盐水（PBS）、脂肪酶和胰蛋白酶溶液中分析水解降解。在PBS和胰蛋白酶中37天期间未检测到质量变化，而观察到脂肪酶的显著降解。将人胎儿包皮成纤维细胞接种在纤维垫和膜上。通过比色细胞活力测定和荧光成像形态学评价群体。基质支持细胞粘附和增殖。新型明胶基聚（氨酯脲）纤维垫为软组织工程应用提供了有吸引力的物理化学和生物学性能。
Biodegradable polyurethanes have been studied as scaffolds for tissue engineering due to their adjustable physico-chemical properties. In this work, we synthesized a biodegradable gelatin-based poly(urethane urea) using polycaprolactone-diol, as soft segment, and isophorone diisocyanate and gelatin from cold water fish skin as hard segment. The synthesis was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance and the influence of the amount of gelatin introduced in the polymer backbone was analyzed by thermal analysis. Gelatin-based poly(urethane urea) electrospun fibrous mats and solvent cast films were then produced and their physico-chemical and biological properties studied. They present an amorphous structure, elastomeric behavior and water contact angles typical of hydrophobic surfaces. Hydrolytic degradation was analyzed in phosphate buffer saline (PBS), lipase and trypsin solutions. No mass changes were detected during 37 days in PBS and trypsin while significant degradation by lipase was observed. Human foetal foreskin fibroblasts were seeded on the fibrous mats and films. Populations were evaluated by colorimetric cell viability assays and morphology by fluorescence imaging. The substrates supported cell adhesion and proliferation. The novel gelatin-based poly(urethane urea) fibrous mats offer attractive physico-chemical and biological properties for soft tissue engineering applications.