Scleral cross-linking using riboflavin and ultraviolet-A radiation for prevention of progressive myopia in a rabbit model
Scleral cross-linking using riboflavin and ultraviolet-A radiation for prevention of progressive myopia in a rabbit model
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DOI：
10.1016/j.exer.2014.07.019
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发表时间：
2014-10-01
影响因子：
3.4
通讯作者：
Bourla, Dan
中科院分区：
文献类型：
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作者：
Dotan, Assaf;Kremer, Israel;Bourla, Dan
我们的研究证实了使用核黄素和紫外线-A辐射的巩膜交联剂对兔模型中轴性近视的发展的影响。用A超对22只13日龄新西兰大白兔的眼球轴长进行了测量。右眼行360度结膜腹膜切开术(实验组，n=11)或不做巩膜交联术(对照组，n=11)，然后进行巩膜缝合术。左眼作为对照眼。实验组将右侧眼球分成象限，每象限设2个(n=8)或6个(n=3)巩膜照射区，每区面积0.2 cm(2)，半径4 mm。用0.1%不含葡聚糖的核黄素-5-磷酸在紫外线照射前20 S和照射时间内每隔20 S滴入一次，进行交联化。UVA照射(370 Nm)垂直于巩膜，功率为57 mW/cm(2)(UVA总剂量为57 J/cm(2))。对照组右眼眼轴长度从基线时的10.50±0.67 mm增加到55天后的15.69±0.39 mm，平均变化5.19±0.85 mm。试验组分别为10.68±0.74 mm和14.29±0.3 mm，平均变化3.61±0.76 mm。两组间平均眼轴长度变化的差异有统计学意义(p<；0.001，Mann-Whitney非参数检验)。目前的文献表明，用核黄素和紫外线A辐射进行巩膜交联术有效地防止了咬合诱导的兔模型眼轴延长。(C)2014爱思唯尔有限公司。保留所有权利。
Our study demonstrates the effect of scleral cross-linking using riboflavin and ultraviolet-A radiation on the development of axial myopia in a rabbit model. Axial length of the eyeball was measured by A-scan ultrasound in 22 New Zealand white rabbits aged 13 days. The right eyes then underwent 360-degree conjunctival peritomy with (experimental group, n = 11) or without (control group, n = 11) scleral cross-linking, followed by tarsorrhaphy. The left eyes served as a control eye. In the experimental group, the right eyeballs were divided into quadrants, and every quadrant had either 2 (n = 8) or 6 (n = 3) scleral irradiation zones, each with an area of 0.2 cm(2) and radius of 4 mm. Cross-linking was performed by dropping 0.1% dextran-free riboflavin-5-phosphate onto the irradiation zones at 20 s before ultraviolet-A irradiation and every 20 s during the 200-s irradiation time. UVA radiation (370 nm) was applied perpendicular to the sclera at 57 mW/cm(2) (total UVA light dose, 57 J/cm(2)). Tarsorrhaphies were removed on day 55, followed by repeated axial-length measurement.In the control group, mean axial length in the right eyes increased from 10.50 +/- 0.67 mm at baseline to 15.69 +/- 039 mm 55 days later, for a mean change of 5.19 +/- 0.85 mm. In the experimental group, corresponding values were 10.68 +/- 0.74 mm and 14.29 +/- 0.3 mm, for a mean change of 3.61 +/- 0.76 mm. The between-group difference in the change in mean axial length was statistically significant (p < 0.001, Mann-Whitney nonparametric test).The present manuscript demonstrates that scleral cross-linking with riboflavin and ultraviolet-A radiation effectively prevents occlusion-induced axial elongation in a rabbit model. (C) 2014 Elsevier Ltd. All rights reserved.