Transient receptor potential channel M5 and phospholipaseC-2 colocalizing in zebrafish taste receptor cells
Transient receptor potential channel M5 and phospholipaseC-2 colocalizing in zebrafish taste receptor cells
复制标题
DOI：
10.1097/wnr.0b013e3282ec6874
复制
发表时间：
2007-10
期刊：
影响因子：
1.7
通讯作者：
Yuki Yoshida;K. Saitoh;Yoshiko Aihara;Shinji Okada;T. Misaka;K. Abe
中科院分区：
文献类型：
--
作者：
Yuki Yoshida;K. Saitoh;Yoshiko Aihara;Shinji Okada;T. Misaka;K. Abe
在哺乳动物中，瞬时受体电位通道M5(Trpm5)与磷脂酶C-2(PLC-2)在味觉感受器细胞中共表达，PLC-2和Trpm5都是甜味、苦味和鲜味刺激信号转导中的重要元件。在本研究中，我们鉴定了斑马鱼Trpm5的同源物(ZfTRPM5)，并通过原位杂交检测了其在味觉系统中的表达。利用在PLC-2启动子控制下表达绿色荧光蛋白的转基因斑马鱼品系，我们证明了zfTRPM5在味蕾绿色荧光蛋白标记的细胞中表达。这些结果表明，zfTRPM5和PLC-2共同定位于斑马鱼的味觉感受器细胞，提示它们通过鱼类味觉感受器在味觉信号转导中发挥重要作用。
In mammals, transient receptor potential (TRP) channel M5 (TRPM5) is coexpressed with phospholipaseC-2 (PLC-2) in the taste receptor cells, and both PLC-2 and TRPM5 are essential elements in the signal transduction of sweet, bitter and umami stimuli. In this study, we identified the zebrafish homologue of TRPM5 (zfTRPM5) and examined its expression in the gustatory system by in-situ hybridization. Using a transgenic zebrafish line that expressed green fluorescent protein under the control of the PLC-2 promoter, we showed that zfTRPM5 is expressed in green fluorescent protein-labeled cells of the taste buds. These results demonstrate that zfTRPM5 and PLC-2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors.