Gas chromatography-mass spectrometry analysis on compounds in volatile oils extracted from Yuan Zhi (Radix Polygalae) and Shi Chang Pu (Acorus Tatarinowii) by supercritical CO2
Gas chromatography-mass spectrometry analysis on compounds in volatile oils extracted from Yuan Zhi (Radix Polygalae) and Shi Chang Pu (Acorus Tatarinowii) by supercritical CO2
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DOI：
10.1016/s0254-6272(13)60055-2
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发表时间：
2012-09-15
影响因子：
2.6
通讯作者：
Liu, Yaming
中科院分区：
文献类型：
--
作者：
Wang, Yingli;Chang, Liping;Liu, Yaming
目的：目的：分析远志、石菖蒲及其混合物的挥发油成分。方法：采用CO2超临界流体萃取法（SFE）提取远志、石菖蒲挥发油，用气相色谱-质谱联用（GC-MS）分析挥发油成分。结果：最佳SEE条件为45 MPa，35 ℃，2 h。从远志石菖蒲混合物中鉴定出24种化合物，其中6种化合物相对含量大于1。这些化合物为1，2-二甲氧基-4-（2-丙烯基）-苯; 1，2，3-三甲氧基-5-（2-丙烯基）-苯; β-细辛醚;（Z，Z）9，1,2-十八碳二烯酸;（Z）6-十八碳烯酸;和油酸乙酯。与单味药相比，中草药配伍后，提取物中抗肿瘤活性物质的数量增加，含1个苯环的化合物的数量减少。结论：中草药中的化合物之间存在协同作用。(C)2012年JTCM。All rights reserved.
OBJECTIVE: To analyze the constituents of volatile oils extracted from Yuan Zhi (Radix Polygalae), Shi Chang Pu (Acorus Tatarinowii), and a mixture of the two herbs.METHODS: The volatile oils were extracted using supercritical fluid extraction (SFE) with CO2, and the constituents of the volatile oil extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). The relative content of each component was calculated using peak area normalization.RESULTS: The optimized SEE conditions were 45 MPa at 35 degrees C for 2 h. Twenty-four compounds were identified in the extract from the Yuan Zhi (Radix Polygalae) and Shi Chang Pu (Acorus Tatarinowii) mixture, and six of these had relative contents >1. These compounds were 1,2-dimethoxy-4-(2-propenyl)-benzene; 1,2,3-trimethoxy-5-(2-propenyl)-benzene; beta-asarone; (Z,Z) 9,12-octadecadienoic acid; (Z) 6-octadecenoic acid; and ethyl oleate. Combination of the herbs increased the number of pharmacologically active substances in the extract and decreased the number of compounds with one benzene ring compared with the extracts from the individual herbs.CONCLUSION: These results indicate there is a synergistic relationship among the compounds in these herbs. (C) 2012 JTCM. All rights reserved.