Strategic Advances in Formation of Cell-in-Shell Structures: From Syntheses to Applications
Strategic Advances in Formation of Cell-in-Shell Structures: From Syntheses to Applications
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DOI：
10.1002/adma.201706063
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发表时间：
2018-04-05
影响因子：
29.4
通讯作者：
Choi, Insung S.
中科院分区：
文献类型：
--
作者：
Kim, Beom Jin;Cho, Hyeoncheol;Choi, Insung S.
单细胞纳米封装，形成细胞在壳结构，提供化学工具，赋予活细胞，在一个程序化的方式，既不是先天的，也不是自然可实现的外源性特性，如级联有机催化，紫外线过滤，免疫原性屏蔽，并在现实生活中的致命因素在体外增强耐受性。该领域的最新进展使得有可能进一步微调包裹单个活细胞的人造壳的物理化学性质，包括按需降解性和可重构性。除了聚电解质之外，许多不同的材料已经被用作细胞涂层材料，适当选择合成策略以将细胞壳结构的潜在应用扩展到全细胞催化和传感器、细胞治疗、组织工程、益生菌包装等。除了传统的“一次性”化学形成的细胞保护，耐用的外壳，自主的，动态的壳化的方法最近也尝试模仿自然发生的孢子形成过程，并使人工壳积极响应和动态。在这里，最近的发展的合成策略，形成细胞在壳结构沿着获得先进的壳性能。示范应用，如全细胞生物催化和细胞治疗，进行了讨论，然后由单细胞纳米封装领域的观点。
Single-cell nanoencapsulation, forming cell-in-shell structures, provides chemical tools for endowing living cells, in a programmed fashion, with exogenous properties that are neither innate nor naturally achievable, such as cascade organic-catalysis, UV filtration, immunogenic shielding, and enhanced tolerance in vitro against lethal factors in real-life settings. Recent advances in the field make it possible to further fine-tune the physicochemical properties of the artificial shells encasing individual living cells, including on-demand degradability and reconfigurability. Many different materials, other than polyelectrolytes, have been utilized as a cell-coating material with proper choice of synthetic strategies to broaden the potential applications of cell-in-shell structures to whole-cell catalysis and sensors, cell therapy, tissue engineering, probiotics packaging, and others. In addition to the conventional "one-time-only" chemical formation of cytoprotective, durable shells, an approach of autonomous, dynamic shellation has also recently been attempted to mimic the naturally occurring sporulation process and to make the artificial shell actively responsive and dynamic. Here, the recent development of synthetic strategies for formation of cell-in-shell structures along with the advanced shell properties acquired is reviewed. Demonstrated applications, such as whole-cell biocatalysis and cell therapy, are discussed, followed by perspectives on the field of single-cell nanoencapsulation.