Brain-derived neurotrophic factor prevents dendritic retraction of adult mouse retinal ganglion cells.
Brain-derived neurotrophic factor prevents dendritic retraction of adult mouse retinal ganglion cells.
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DOI：
10.1111/ejn.13295
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Morgan JE
中科院分区：
文献类型：
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作者：
Binley KE;Ng WS;Barde YA;Song B;Morgan JE
我们使用培养的成年小鼠视网膜作为模型系统，使用视网膜神经节细胞（RGC）的diolistic标记，跟踪和量化树突的回缩。通过核染色平行监测细胞死亡，因为用RGC和凋亡标志物“标记”是不一致的，并且非常难以可靠地定量。核染色使我们能够描绘一个漫长的时间窗口，在此期间，可以在没有RGC死亡的情况下监测树突回缩。添加脑源性神经营养因子（BDNF）可显著减少树突状变性，即使在视网膜脱离后延迟3天应用。这些结果表明，延迟添加营养因子可能在青光眼等疾病过程中细胞体损失之前具有功能上的益处。
We used cultured adult mouse retinae as a model system to follow and quantify the retraction of dendrites using diolistic labelling of retinal ganglion cells (RGCs) following explantation. Cell death was monitored in parallel by nuclear staining as ‘labelling’ with RGC and apoptotic markers was inconsistent and exceedingly difficult to quantify reliably. Nuclear staining allowed us to delineate a lengthy time window during which dendrite retraction can be monitored in the absence of RGC death. The addition of brain‐derived neurotrophic factor (BDNF) produced a marked reduction in dendritic degeneration, even when application was delayed for 3 days after retinal explantation. These results suggest that the delayed addition of trophic factors may be functionally beneficial before the loss of cell bodies in the course of conditions such as glaucoma.