Major outer membrane proteins from Porphyromonas gingivalis:: strain variation, distribution, and clinical significance in periradicular lesions
Major outer membrane proteins from Porphyromonas gingivalis:: strain variation, distribution, and clinical significance in periradicular lesions
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DOI：
10.1111/j.1600-0722.2005.00235.x
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发表时间：
2005-10-01
影响因子：
1.9
通讯作者：
Yoshimura, F
中科院分区：
文献类型：
--
作者：
Imai, M;Murakami, Y;Yoshimura, F
牙龈卟啉单胞菌与边缘性牙周炎和根尖周感染有关。本研究检测了牙龈卟啉单胞菌与根周病变相关的主要外膜蛋白。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和N-末端氨基酸分析比较了牙龈卟啉单胞菌ATCC 33277和W83的外膜蛋白谱。大多数外膜蛋白，如RagA，牙龈菌蛋白酶，和OmpA样蛋白，被发现在这两个菌株在一个类似的分布模式，但是，迁移位置的赖氨酸牙龈菌蛋白酶和RagB在SDS-PAGE中被倒置。Western印迹分析表明，RagA，RagB和OmpA样蛋白在所有测试的牙龈卟啉单胞菌菌株中被发现。W83抗RagB的抗血清对某些菌株如ATCC 33277的反应较差。当检查与牙龈卟啉单胞菌遗传学相关的菌株时，在几种菌株中免疫学检测到RagA和OmpA同源物。然而，在所分析的任何菌株中均未检测到RagB同源物，表明RagB是牙龈卟啉单胞菌所特有的。为了检查牙龈卟啉单胞菌中的免疫反应性抗原，使用了来自根周病变患者的血清。超过一半的血清显示对牙龈卟啉单胞菌细胞组分的强烈反应，尤其是RagB。我们的研究结果表明，一个主要的外膜蛋白，RagB，是一个可能的毒力因子在根周病变。
Porphyromonas gingivalis has been implicated in both marginal periodontitis and periapical infection. This study examined the major outer membrane proteins, from P. gingivalis, which related to periradicular lesions. Outer membrane protein profiles of P. gingivalis ATCC 33277 and W83 were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid analysis. Most outer membrane proteins, such as RagA, gingipains, and OmpA-like proteins, were found in both strains in a similar distribution pattern; however, the migration positions of Lys-gingipain and RagB were inverted in SDS-PAGE. Western blot analysis showed that RagA, RagB, and OmpA-like proteins were found in all of the P. gingivalis strains tested. The antiserum of W83 against RagB reacted poorly to some strains, such as ATCC 33277. When strains phylogenetically related to P. gingivalis were examined, RagA and OmpA homologs were immunologically detected in several strains. However, none of the RagB homologs were detected in any strain analyzed, suggesting that RagB is unique to P. gingivalis. To examine immunoreactive antigens in P. gingivalis, sera from patients with periradicular lesions were used. More than half of the sera showed strong reactions to P. gingivalis cell components, especially RagB. Our results indicate that a major outer membrane protein, RagB, is a possible virulence factor in periradicular lesions.