Genetic diversity of Sinojackia dolichocarpa (Styracaceae), a species endangered and endemic to China, detected by inter-simple sequence repeat (ISSR)
Genetic diversity of Sinojackia dolichocarpa (Styracaceae), a species endangered and endemic to China, detected by inter-simple sequence repeat (ISSR)
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DOI：
10.1016/j.bse.2005.11.001
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发表时间：
2006-03-01
影响因子：
1.6
通讯作者：
Jin, XF
中科院分区：
文献类型：
--
作者：
Cao, PJ;Yao, QF;Jin, XF
长果秤锤木是我国特有的濒危植物，仅分布于湖南省石门、桑植一带。利用简单重复序列（Inter-simple sequence repeat，ISSR）分子标记技术，对四个野生居群的遗传多样性进行了研究。长果从84个个体中收集了叶样品。从80个引物中筛选出13个引物，共扩增出137条DNA带，其中多态性条带100条，占72.99%。平均每个引物扩增出10.5条带，其中多态性条带7.7条。在物种水平上，检测到较高的遗传多样性（PPB：72.99%; H-E：0.2255; Ho：0.3453）。然而，相对较低的遗传多样性存在于群体内。毛竹河种群的变异性最大（PPB：40.38%，H-E：0.1566，Ho：0.2330），而京官门种群的变异性最小（PPB：30.66%，H-E：0.1078，Ho：0.1601）。Nei的基因多样性统计（45.30%）、Shannon的信息量（45.24%）和分子方差分析（AMOVA）均揭示了居群间的高度遗传分化（52.88%）。居群间高度分化的主要原因可能与自交生殖系统和居群隔离有关。居群间较强的遗传分化表明，对遗传多样性的保护需要加强管理。长果派应该致力于保护每一个种群(c)2005爱思唯尔有限公司保留所有权利。
Sinojackia dolichocarpa, a species endangered and endemic to China, is distributed only in the regional area of Shimen and Sangzhi in Hunan Province. Inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity within and among the four natural populations of S. dolichocarpa. Leaf samples were collected from 84 individuals. Thirteen ISSR primers selected from 80 primers gave rise to 137 discernible DNA bands of which 100 (72.99%) were polymorphic. On average each primer gave rise to 10.5 bands including 7.7 bands with polymorphic profile. At the species level, high genetic diversity was detected (PPB: 72.99%; H-E: 0.2255; Ho: 0.3453). However, relatively low genetic diversity existed within populations. Population in Maozhuhe (MZH) exhibits the greatest level of variability (PPB: 40.38%, H-E: 0.1566, Ho: 0.2330), whereas the population in Jingguanmen (JGM) finds its own variability at the lowest level (PPB: 30.66%; H-E: 0.1078; Ho: 0.1601). A high level of genetic differentiation among populations was revealed by Nei's gene diversity statistics (45.30%), Shannon's information measure (45.24%) and analysis of molecular variance (AMOVA) (52.88%). The main factors responsible for the high level of differentiation among populations are probably related to the selfing reproductive system and the isolation of populations. The strong genetic differentiation among populations indicates that the management for the conservation of genetic variability in S. dolichocarpa should aim to preserve every population. (c) 2005 Elsevier Ltd. All rights reserved.