DETERGENT COMPONENTS IN SEWAGE EFFLUENT ARE WEAKLY ESTROGENIC TO FISH - AN IN-VITRO STUDY USING RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) HEPATOCYTES
DETERGENT COMPONENTS IN SEWAGE EFFLUENT ARE WEAKLY ESTROGENIC TO FISH - AN IN-VITRO STUDY USING RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) HEPATOCYTES
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DOI：
10.1016/0166-445x(93)90064-8
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发表时间：
1993-12-01
影响因子：
4.5
通讯作者：
SUMPTER, JP
中科院分区：
文献类型：
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作者：
JOBLING, S;SUMPTER, JP
烷基酚聚乙氧基化物（APnEO，n 1-40）是一种主要的表面活性剂，通常存在于原污水中。这些化合物生物降解的许多产物具有持久性，并大量存在于污水和河水中。我们在这里报告使用体外生物测定，以确定这些化合物的雌激素效力的鱼。生物测定是基于肝细胞合成卵黄蛋白原是雌激素依赖性的事实。在测试的化合物中，4-壬基苯酚、4-叔辛基苯酚、4-叔丁基苯酚、4-壬基苯酚-二乙氧基化物、Tergitol-NP 9和4-壬基苯氧基羧酸都是弱雌激素，效力在17 β-雌二醇活性的约1 × 10（-4）至1 × 10（-6）之间。观察到的雌激素活性似乎仅限于帕拉或4取代化合物，因为2-叔丁基苯酚和3-叔丁基苯酚是无活性的。随着乙氧基链长度的增加，聚乙氧基化物化合物的雌激素性降低。因此，暴露于Tergitol NP 40 EO（具有40的链长）的细胞不分泌卵黄蛋白原。同时暴露的肝细胞他莫昔芬（一种雌激素拮抗剂）和有效剂量的代表性化合物引起的雌激素效应的抑制在所有情况下，这表明这些化合物的作用是由雌二醇受体介导的。
Alkylphenol-polyethoxylates (APnEO, n 1-40) are a major group of surfactants and are normally present in raw sewage. Many of the products of the biodegradation of these compounds are both persistent and present in substantial quantities in effluent and in river water. We report here on the use of an in vitro bioassay to determine the oestrogenic potencies of these compounds to fish. The bioassay is based on the fact that the synthesis of vitellogenin by hepatocytes is oestrogen dependent. Of the compounds tested, 4-nonylphenol, 4-tert-octylphenol, 4-tert-butylphenol 4-nonylphenol-diethoxylate, Tergitol-NP9, and 4-nonylphenoxycarboxylic acid were all weakly oestrogenic, with potencies between about 1 x 10(-4) to 1 x 10(-6) the activity of 17 beta-oestradiol The oestrogenic activity observed appeared to be confined to para or 4 substituted compounds, because 2-tert-butylphenol and 3-tert-butylphenol were inactive. The polyethoxylate compounds became less oestrogenic with increasing lengh of the ethoxy chain. Thus cells exposed to Tergitol NP40EO (with a chain length of 40) did not secrete vitellogenin. Simultaneous exposure of the hepatocytes to Tamoxifen (an oestrogen antagonist) and effective doses of representative compounds caused an inhibition of the oestrogenic effect in all cases, suggesting that the action of these compounds is mediated by the oestradiol receptor.