Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.
Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.
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DOI：
10.1126/sciadv.abq7352
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发表时间：
2022-11-18
期刊：
影响因子：
13.6
通讯作者：
Jarvis, R. Paul
中科院分区：
文献类型：
--
作者：
Sun, Yi;Yao, Zujie;Ye, Yiting;Fang, Jun;Chen, Honglin;Lyu, Yuping;Broad, William;Fournier, Marjorie;Chen, Genyun;Hu, Yonghong;Mohammed, Shabaz;Ling, Qihua;Jarvis, R. Paul
光合作用是地球上大多数生命的能量基础，在植物中，光合作用在称为叶绿体的双膜细胞器内进行。光合作用装置包含许多由核和细胞器基因组编码的蛋白质。该装置的维护需要内部叶绿体蛋白酶的作用，但由于双膜包膜存在屏障，预计核胞质泛素蛋白酶体系统（UPS）不会发挥作用。在这里，我们表明光合作用蛋白（包括由叶绿体基因内部编码的蛋白）通过 CHLORAD 途径泛素化和加工：它们在 CDC48 依赖性逆转录至胞质溶胶后被 26S 蛋白酶体降解。这表明 UPS 的作用范围延伸到内共生衍生的叶绿体的内部，在那里它起到调节光合作用的作用，这可以说是生命最基本的过程。泛素蛋白酶体系统以叶绿体内的蛋白质为目标，包括叶绿体编码的光合作用亚基。
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double membrane–bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected, owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated and processed via the CHLORAD pathway: They are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically derived chloroplasts, where it acts to regulate photosynthesis, arguably the most fundamental process of life. The ubiquitin-proteasome system targets proteins inside chloroplasts, including chloroplast-encoded photosynthesis subunits.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
3.5
作者：
Aronsson, H;Jarvis, P
通讯作者：
Jarvis, P
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
DOI：
10.1007/978-1-61779-234-2_21
发表时间：
2011-01-01
期刊：
CHLOROPLAST RESEARCH IN ARABIDOPSIS: METHODS AND PROTOCOLS, VOL I
影响因子：
--
作者：
Froehlich, John
通讯作者：
Froehlich, John
影响因子：
16.6
作者：
Grimmer, Julia;Helm, Stefan;Baginsky, Sacha
通讯作者：
Baginsky, Sacha