Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues
Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues
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DOI：
10.1016/s0169-328x(97)00071-5
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发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Howard, AD
中科院分区：
文献类型：
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作者：
Guan, XM;Yu, H;Howard, AD
生长激素的释放受到两种下丘脑激素的严格控制：生长激素释放激素和生长抑素。此外，合成的生长激素促分泌剂也已被证明可通过生长激素促分泌素受体（GHS - R）调节生长激素的释放，这表明存在另一种生长激素释放的生理调节因子。为了更详细地了解GHS - R的生理作用，我们利用大鼠和人体组织通过原位杂交和核糖核酸酶保护分析对该受体的mRNA表达进行了定位。在大鼠脑中，主要信号在多个下丘脑核以及垂体中被检测到。在海马结构的齿状回也观察到强烈信号。其他显示该受体局部和离散信号的脑区包括海马的CA2和CA3区域、黑质、腹侧被盖区以及中缝背核和中缝正中核。与大鼠脑的结果相似，利用人体组织进行的核糖核酸酶保护分析在垂体、下丘脑和海马中显示出特定信号。此外，在胰腺中也注意到一个微弱信号。GHS - R在下丘脑和垂体的定位证明与其在调节生长激素释放中的作用是一致的。该受体在其他中枢和外周区域的表达可能暗示其参与其他尚未明确的生理功能。（C）1997年爱思唯尔科学出版社（Elsevier Science B.V.）
Growth hormone release is under tight control by two hypothalamic hormones: growth hormone-releasing hormone and somatostatin. In addition, synthetic growth hormone secretagogues have also been shown to regulate growth hormone release through the growth hormone secretagogue receptor (GHS-R), suggesting the existence of an additional physiological regulator for growth hormone release. To understand the physiological role of the GHS-R in more detail, we mapped the expression of mRNA for the receptor by in situ hybridization and RNase protection assays using rat and human tissues. In the rat brain, the major signals were detected in multiple hypothalamic nuclei as well as in the pituitary gland. Intense signals were also observed in the dentate gyrus of the hippocampal formation. Other brain areas that displayed localized and discrete signals for the receptor include the CA2 and CA3 regions of the hippocampus, the substantia nigra, ventral tegmental area, and dorsal and median raphe nuclei. In resemblance to the results from rat brain, RNase protection assays using human tissues revealed specific signals in pituitary, hypothalamus and hippocampus. Moreover, a weak signal was noted in the pancreas. The demonstration of hypothalamic and pituitary localization of the GHS-R is consistent with its role in regulating growth hormone release. The expression of the receptor in other central and peripheral regions may implicate its involvement in additional as yet undefined physiological functions. (C) 1997 Elsevier Science B.V.