Translating biomolecular recognition into nanomechanics
Translating biomolecular recognition into nanomechanics
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DOI：
10.1126/science.288.5464.316
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发表时间：
2000-04-14
期刊：
影响因子：
56.9
通讯作者：
Gimzewski, JK
中科院分区：
文献类型：
--
作者：
Fritz, J;Baller, MK;Gimzewski, JK
我们报告的具体转导，通过表面应力的变化，DNA杂交和受体-配体结合成一个直接的纳米机械响应的微制造的杠杆。阵列中的悬臂用选择的生物分子功能化。发现尽管单个杠杆的非特异性响应较大，但杠杆的差异偏转提供了真正的分子识别信号。互补寡核苷酸的杂交显示，两个12聚体寡核苷酸之间的单碱基错配是明确可检测的。蛋白A-免疫球蛋白相互作用的类似实验证明了纳米机械转导检测生物分子识别的广泛适用性。
We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite Large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.