Phase transition of GvpU regulates gas vesicle clustering in bacteria.
Phase transition of GvpU regulates gas vesicle clustering in bacteria.
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DOI：
10.1038/s41564-024-01648-3
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发表时间：
2024-03
影响因子：
28.3
通讯作者：
Zongru Li;Qionghua Shen;E. T. Usher;Andrew P Anderson;Manuel Iburg;Richard Lin;Brandon Zimmer;M. D. Meyer;A. Holehouse;Lingchong You;Ashutosh Chilkoti;Yifan Dai;George J Lu
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作者：
Zongru Li;Qionghua Shen;E. T. Usher;Andrew P Anderson;Manuel Iburg;Richard Lin;Brandon Zimmer;M. D. Meyer;A. Holehouse;Lingchong You;Ashutosh Chilkoti;Yifan Dai;George J Lu
气体囊泡 (GV) 是支持细胞浮力的微生物蛋白质细胞器。 GV 工程有多种应用，包括报告基因成像、声学控制和有效载荷传输。 GV 通常聚集成蜂窝状，以尽量减少对细胞质的占用。潜在的分子机制和对细胞生理学的影响仍然未知。利用遗传、生化和成像方法，我们从 Priestia megaterium 中鉴定出 GvpU 作为一种在体外和在大肠杆菌中表达时调节 GV 聚类的蛋白质。 GvpU 与核心 GV 壳蛋白的 C 末端尾部结合，并经历相变，在不饱和溶液中形成簇。 GvpU 的这些特性可调节 GV 聚类并直接调节细菌适应性。 GV 变体可以设计为对 GvpU 介导的聚类具有可控的敏感性，从而能够设计遗传可调的生物传感器。我们的研究结果阐明了 GV 聚类的分子机制和功能作用，使其能够在生物医学应用中具有可编程性。
Gas vesicles (GVs) are microbial protein organelles that support cellular buoyancy. GV engineering has multiple applications, including reporter gene imaging, acoustic control and payload delivery. GVs often cluster into a honeycomb pattern to minimize occupancy of the cytosol. The underlying molecular mechanism and the influence on cellular physiology remain unknown. Using genetic, biochemical and imaging approaches, here we identify GvpU fromPriestia megateriumas a protein that regulates GV clustering in vitro and upon expression inEscherichia coli. GvpU binds to the C-terminal tail of the core GV shell protein and undergoes a phase transition to form clusters in subsaturated solution. These properties of GvpU tune GV clustering and directly modulate bacterial fitness. GV variants can be designed with controllable sensitivity to GvpU-mediated clustering, enabling design of genetically tunable biosensors. Our findings elucidate the molecular mechanisms and functional roles of GV clustering, enabling its programmability for biomedical applications.