Coordinate recruitment of E-cadherin and ALCAM to cell-cell contacts by alpha-catenin.
Coordinate recruitment of E-cadherin and ALCAM to cell-cell contacts by alpha-catenin.
复制标题
通过 α-连环蛋白协调 E-钙粘蛋白和 ALCAM 募集至细胞间接触。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
J. Schalken
中科院分区：
文献类型：
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作者：
K. Tomita;A. Bokhoven;C. Jansen;M. Bussemakers;J. Schalken
在这里，我们报告的作用，α-连环蛋白在细胞定位的活化白细胞粘附分子，ALCAM，钙粘蛋白介导的细胞粘附在人前列腺癌细胞。具有功能性E-钙粘蛋白介导的细胞粘附的细胞系（DU-145和LNCaP）在细胞-细胞接触处显示ALCAM染色。相反，在缺乏α-连环蛋白表达的细胞系（阿尔瓦-31、PC-3和PPC-1）中，E-钙粘蛋白介导的粘附受到干扰，ALCAM染色为胞质。通过将α-N-连环蛋白构建体转染到细胞系阿尔瓦-31和PC-3中，确定了α-连环蛋白在募集E-钙粘蛋白和ALCAM至细胞-细胞接触中的作用。这不仅导致E-钙粘蛋白/α-连环蛋白复合物在细胞膜上的正确组装，而且还导致ALCAM定位于细胞-细胞接触，表明α-连环蛋白确实影响ALCAM定位。
Here we report on the role of alpha-catenin in the cellular localization of activated leukocyte cell adhesion molecule, ALCAM, and cadherin-mediated cell adhesion in human prostate cancer cells. Cell lines that have a functional E-cadherin-mediated cell adhesion (DU-145 and LNCaP) show ALCAM staining at cell-cell contacts. In contrast, in cell lines that lack alpha-catenin expression (ALVA-31, PC-3, and PPC-1), E-cadherin-mediated adhesion is disturbed and ALCAM staining is cytoplasmic. A role of alpha-catenin in the recruitment of E-cadherin and ALCAM to cell-cell contacts was established by transfection of an alpha-N-catenin construct into cell lines ALVA-31 and PC-3. This resulted not only in the correct assembly of E-cadherin/alpha-catenin complexes at the cell membrane but also in localization of ALCAM to cell-cell contacts, indicating that indeed alpha-catenin affects ALCAM localization.
影响因子：
2.7
作者：
Frenzel, EM;Johnson, RG
通讯作者：
Johnson, RG
影响因子：
3.3
作者：
Theard, Delphine;Raspe, Marcel A.;van IJzendoorn, Sven C. D.
通讯作者：
van IJzendoorn, Sven C. D.