Characterization of Phenol Metabolization by P. stutzeri N2
Characterization of Phenol Metabolization by P. stutzeri N2
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DOI：
10.1080/10406638.2015.1033434
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发表时间：
2016-10
影响因子：
2.4
通讯作者：
Hongyun Nie;M. Nie;Yong-Zhe Yang;Jing Zhao-;Xinyi Zhang;Yutao Guo;Yi Wan;Jing Zi
中科院分区：
文献类型：
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作者：
Hongyun Nie;M. Nie;Yong-Zhe Yang;Jing Zhao-;Xinyi Zhang;Yutao Guo;Yi Wan;Jing Zi
通过对邻苯二酚1，2-加氧酶和邻苯二酚2，3-双加氧酶相关代谢产物和酶活性的鉴定，初步探讨了斯氏原杆菌N2对苯酚的代谢特性。施氏原杆菌N2在400 mg l-1苯酚上开始生长，没有明显的滞后期，然后在32 h内迅速完成苯酚分解。酶活测定、紫外光谱分析、顺式、顺式粘康酸和2-HMS质谱鉴定结果表明，邻苯二酚C12 O和C23 O同时作用于酚环的断裂。对羟基苯甲酸和直链饱和二元羧酸的存在表明，苯酚的降解过程中涉及羧化和氢化。含有5个或4个碳的代谢物表明邻苯二酚及其直接开环中间体可以进一步被施氏原杆菌N2代谢。因此，斯氏毕赤酵母N2降解苯酚的高效性和途径多样性在含酚废水处理中具有潜在的应用价值。
Characterization of phenol metabolization by P. stutzeri N2 was proposed based on the identification of relevant metabolites and enzyme activities of catechol 1,2-oxygenase and catechol 2,3-dioxygenase. P. stutzeri N2 started to grow on 400 mg l−1 phenol with no obvious lag phase, and then phenol decomposition was rapidly completed within 32 h. Results of enzyme activities, UV spectrums of cultures, and the mass spectrum identification of cis, cis-muconic and 2-HMS, which showed that catechol C12O and C23O were acting simultaneously on the fission of the phenol ring. The existence of P-hydroxybenzoic acid and straight-chain saturated dicarboxylic acids in the culture medium showed that carboxylation and hydrogenation were involved during the phenol degradation. Metabolites containing five or four carbons indicated that catechol and its immediate ring-cleavage intermediates could be furthermore metabolized by P. Stutzeri N2. Therefore, P. stutzeri N2 which could degrade phenol with high efficiency and diverse pathways could have potential applications in the treatment of wastewater containing phenol.