Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts
Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts
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DOI：
10.1128/jvi.01917-07
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发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
Tsurumi, Tatsuya
中科院分区：
文献类型：
--
作者：
Isomura, Hiroki;Stinski, Mark F.;Tsurumi, Tatsuya
在生产性感染过程中，人巨细胞病毒（HCMV）UL44转录起始于三个不同的起始位点，这些位点受到差异性调节。两个起始位点，远端和近端，在早期是活跃的，而中间的起始位点仅在感染后的晚期才活跃。UL44早期病毒基因产物是病毒DNA合成所必需的。来自晚期病毒启动子的UL44基因产物主要影响感染后晚期的病毒基因表达，而不是病毒DNA合成（H. Isomura，M. F. Stinski，A. Kudoh，S.中山山Iwahori，Y. Sato和T. Tsurumi，J. Virol. 81：6197，2007）。UL44早期病毒启动子具有典型的TATA序列“TATAA.相反，UL44晚期病毒启动子具有非经典的TATA序列。使用重组病毒，我们发现，非经典的TATA序列是需要后期病毒转录本的积累。中间TATA元件周围的GC盒不影响UL44后期转录的动力学或起始位点。用非经典TATA序列替换远端TATA元件不影响转录动力学或转录起始位点，但它确实在感染后的晚期诱导了替代转录物。数据表明，非经典的TATA盒是在HCMV感染后的晚期使用。
During productive infection, human cytomegalovirus (HCMV) UL44 transcription initiates at three distinct start sites that are differentially regulated. Two of the start sites, the distal and the proximal, are active at early times, whereas the middle start site is active only at late times after infection. The UL44 early viral gene product is essential for viral DNA synthesis. The UL44 gene product from the late viral promoter affects primarily viral gene expression at late times after infection rather than viral DNA synthesis (H. Isomura, M. F. Stinski, A. Kudoh, S. Nakayama, S. Iwahori, Y. Sato, and T. Tsurumi, J. Virol. 81:6197, 2007). The UL44 early viral promoters have a canonical TATA sequence, "TATAA." In contrast, the UL44 late viral promoter has a noncanonical TATA sequence. Using recombinant viruses, we found that the noncanonical TATA sequence is required for the accumulation of late viral transcripts. The GC boxes that surround the middle TATA element did not affect the kinetics or the start site of UL44 late transcription. Replacement of the distal TATA element with a noncanonical TATA sequence did not affect the kinetics of transcription or the transcription start site, but it did induce an alternative transcript at late times after infection. The data indicate that a noncanonical TATA box is used at late times after HCMV infection.