Glial progenitors of the neonatal subventricular zone differentiate asynchronously, leading to spatial dispersion of glial clones and to the persistence of immature glia in the adult mammalian CNS.
Glial progenitors of the neonatal subventricular zone differentiate asynchronously, leading to spatial dispersion of glial clones and to the persistence of immature glia in the adult mammalian CNS.
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新生儿室下区的神经胶质祖细胞异步分化，导致神经胶质克隆的空间分散以及成年哺乳动物中枢神经系统中未成熟神经胶质的持续存在。
DOI：
10.1016/j.ydbio.2004.02.024
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Goldman,JamesE
中科院分区：
文献类型：
--
作者：
Zerlin,Marielba;Milosevic,Ana;Goldman,JamesE
哺乳动物前脑发育中的脑室下区(SVZ)在出生时在新皮质和白质中形成星形胶质细胞和少突胶质细胞，在嗅球中产生神经元。我们用逆转录病毒“文库”在出生后第0-1天(P0-1)体内感染单个SVZ细胞的后代，观察了它们的发育命运和空间分布。在大多数情况下，单个SVZ细胞产生少突胶质细胞或星形胶质细胞，但有些细胞同时产生两种类型的胶质细胞。神经胶质克隆的成员可以广泛分散在灰质和白质中。祖细胞在停止迁移后继续分裂，产生相关的细胞团。然而，单个SVZ细胞的后代并不同步分化：单个克隆在短或长时间间隔后既含有成熟的胶质细胞，也含有不成熟的胶质细胞。例如，定居在白质中的祖细胞产生了三种类型的克隆性少突胶质细胞簇：仅由髓鞘少突胶质细胞组成的克隆少突胶质细胞簇，髓鞘少突胶质细胞和非髓鞘少突胶质细胞组成的克隆少突胶质细胞簇，或仅由少突胶质细胞谱系的非髓鞘细胞组成的克隆少突胶质细胞簇。因此，一些祖细胞没有完全分化，但仍然不成熟，可能会继续很好地循环到成年生活。
The subventricular zone (SVZ) of the developing mammalian forebrain gives rise to astrocytes and oligodendrocytes in the neocortex and white matter, and neurons in the olfactory bulb in perinatal life. We have examined the developmental fates and spatial distributions of the descendants of single SVZ cells by infecting them in vivo at postnatal day 0–1 (P0–1) with a retroviral “library”. In most cases, individual SVZ cells gave rise to either oligodendrocytes or astrocytes, but some generated both types of glia. Members of glial clones can disperse widely through the gray and white matter. Progenitors continued to divide after stopping migration, generating clusters of related cells. However, the progeny of a single SVZ cell does not differentiate synchronously: individual clones contained both mature and less mature glia after short or long intervals. For example, progenitors that settled in the white matter generated three types of clonal oligodendrocyte clusters: those composed of only myelinating oligodendrocytes, of both myelinating oligodendrocytes and non-myelinating oligodendrocytes, or of only non-myelinating cells of the oligodendrocyte lineage. Thus, some progenitors do not fully differentiate, but remain immature and may continue to cycle well into adult life.