Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured Müller cells.
Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured Müller cells.
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DOI：
10.3892/mmr.2012.1152
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发表时间：
2013-01
影响因子：
3.4
通讯作者：
Sun JH
中科院分区：
文献类型：
--
作者：
Ai J;Liu Y;Sun JH
越来越多的证据表明，晚期糖基化终产物（AGEs）在糖尿病血管并发症（包括糖尿病视网膜病变（DR））的发展中起着因果作用。为了评估AGEs和碱性成纤维细胞生长因子（bFGF）之间的相互关系，研究了AGEs对Müller细胞产生bFGF的影响。Müller细胞从成年兔视网膜培养。用高度糖化的牛血清白蛋白（BSA）制备AGEs，并在不含葡萄糖的相同条件下孵育对照非糖化BSA（BSA对照）。将培养的Müller细胞在其所需培养基中暴露于AGEs或BSA对照（体积百分比为4、8、16、32和64%）1、3、6和9天的时间过程。免疫细胞化学法检测Müller细胞中bFGF的表达。使用专用软件的计算机化图像分析通过密度测定法进行定量。体积百分比分别为16%、32%和16%、32%、64%的AGEs在第1、3、6、9天均能增加Müller细胞bFGF的表达（P<0.05）。此外，AGEs以时间依赖性方式上调Müller细胞中bFGF的表达。总之，用AGEs处理Müller细胞导致培养基中bFGF的剂量和时间依赖性升高。本研究的结果表明，玻璃体中AGEs形成的增加可能通过诱导视网膜Müller细胞产生bFGF而参与DR的发生。
Accumulating evidence points to a causal role for advanced glycation end-products (AGEs) in the development of diabetic vascular complications, including diabetic retinopathy (DR). To assess the reciprocal correlation between AGEs and basic fibroblast growth factor (bFGF), the effects of AGEs on the production of bFGF by Müller cells were investigated. Müller cells were cultured from adult rabbit retinas. The AGEs were prepared with highly glycated bovine serum albumin (BSA) and the control non-glycated BSA (BSA control) was incubated under the same conditions without glucose. Cultured Müller cells were exposed to AGEs or BSA control (volume percentages were 4, 8, 16, 32 and 64%) for a time course of 1, 3, 6 and 9 days in their desired medium. The expression of bFGF in Müller cells was evaluated by immunocytochemistry. Quantification was performed by densitometry using computerized image analysis with dedicated software. AGEs in a volume percentage of 16 and 32% on day 1 and in a volume percentage of 16, 32 and 64% on days 3, 6 and 9 increased the bFGF expression in Müller cells (P<0.05). Additionally, AGEs upregulated bFGF expression in Müller cells in a time-dependent manner. In conclusion, the treatment of Müller cells with AGEs resulted in a dose- and time-dependent elevation of bFGF in the culture medium. The results from this study suggest that the increased formation of AGEs in the vitreous may be involved in the development of DR by inducing the production of bFGF by retinal Müller cells.
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DOI：
10.2147/opth.s27423
发表时间：
2012
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Baharivand N;Zarghami N;Panahi F;Dokht Ghafari MY;Mahdavi Fard A;Mohajeri A
通讯作者：
Mohajeri A
影响因子：
8.2
作者：
Curtis, T. M.;Hamilton, R.;Stitt, A. W.
通讯作者：
Stitt, A. W.
影响因子：
5.1
作者：
Kakehashi, Akihiro;Inoda, Shigeru;Kanazawa, Yasunori
通讯作者：
Kanazawa, Yasunori
影响因子：
5.2
作者：
Liu, Wenbo;Xu, Jiying;Han, Mingyong
通讯作者：
Han, Mingyong
影响因子：
5.4
作者：
Tokuda, Haruhiko;Adachi, Seiji;Kozawa, Osamu
通讯作者：
Kozawa, Osamu