Evolution of RNA interference proteins dicer and argonaute in Basidiomycota
Evolution of RNA interference proteins dicer and argonaute in Basidiomycota
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DOI：
10.3852/13-171
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发表时间：
2013-11-01
期刊：
影响因子：
2.8
通讯作者：
Olson, Ake
中科院分区：
文献类型：
--
作者：
Hu, Yang;Stenlid, Jan;Olson, Ake
RNA干扰(RNAi)是指细胞控制基因表达，保护基因组免受移动的重复DNA序列、逆转录元件和转座子的影响，并保护自己免受病毒感染的一种机制。在RNAi机制中，有两个核心组件，DICER和ArgAerte是核心。本研究以担子菌为研究对象，以43个真菌基因组为研究对象，分析了Argavite和Dicer基因的进化过程。在担子菌纲和其他真菌类群中广泛存在ArgAerte和Dicer基因，但它们的拷贝数不同。然而，在某些血统中，阿尔加索特或骰子是缺失的。我们的结果表明，在担子菌门早期多样化的同时，Dicer和ArgAerte基因的古代复制，以及更近起源的物种特有的复制和损失。真菌中存在几条不同的RNAi途径，基于结构相似性和系统发育关系，我们的结果表明大多数担子菌可能存在抑制，而在担子菌门中没有发现任何MSUD(减数分裂-沉默)途径的证据。RNAi已发展成为反向遗传学研究的重要工具。由于几乎所有担子菌中都存在ArgAerte和Dicer，我们的结果表明，RNAi应该是可能的，作为研究大多数担子菌门基因功能的工具。
RNA interference (RNAi) refers to a mechanism in which cells control gene expression, protect the genome against mobile repetitive DNA sequences, retro elements and transposons, and defend themself against viruses. Two core components, dicer and argonaute, are central in the RNAi machinery. In this study the evolution of argonaute and dicer genes were analyzed with 43 fungal genomes, with the focus on Basidiomycota. Argonaute and dicer genes are widely represented in Basidiomycota as well as in other fungal groups, but the number of copies of them vary. However, in certain lineages, argonaute or dicer is missing. Our results suggest an ancient duplication of dicer and argonaute genes concurrently with early diversification of the Basidiomycota followed by additional species-specific duplications and losses of more recent origin. Several distinct RNAi pathways exist in fungi, based on structural similarity and phylogenetic relationship, our results indicate that quelling possibly exists in most Basidiomycota, while we could not find any evidence for the MSUD (meiotic-silencing) pathway in Basidiomycota. RNAi has been developed to be an important tool for reverse genetics studies. Because both argonaute and dicer are present in almost all Basidiomycota our results indicate that it should be possible to develop RNAi as a tool for functional studies of genes in most Basidiomycota species.