Ozone alters the expression of tenascin-C in cultured primate nasal epithelial cells.
Ozone alters the expression of tenascin-C in cultured primate nasal epithelial cells.
复制标题
臭氧改变培养的灵长类鼻上皮细胞中生腱蛋白-C 的表达。
DOI：
10.1165/ajrcmb.18.4.2966
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Wight,TN
中科院分区：
文献类型：
--
作者：
Potter-Perigo,S;Kaplan,ED;Luchtel,DL;Baker,C;Altman,LC;Wight,TN
生腱蛋白-C是与上皮细胞脱离、增殖和迁移相关的瞬时表达的细胞外基质组分。这种分子已在呼吸组织中被鉴定，但对生腱蛋白-C的细胞来源或调节其产生的因子知之甚少。由于空气污染物是已知的破坏上皮的完整性，我们研究了调节腱生蛋白-C响应于0.3 ppm的臭氧在分化的灵长类动物鼻上皮细胞培养在空气介质界面。通过北方印迹分析、蛋白质印迹和免疫荧光染色确定，生腱蛋白-C的表达在臭氧的作用下上调。相反，多功能蛋白聚糖、双糖蛋白聚糖、串珠蛋白聚糖或I型胶原蛋白的mRNA水平没有变化。在臭氧处理的培养物中，细胞与基底表面之间的界面处的生腱蛋白-C沉积与细胞与基底表面之间的界面处的细胞附着减少是明显的。在基质的裸露区域上存在腱生蛋白-C表明腱生蛋白-C可能在细胞斑块的损失中起作用。腱生蛋白C的合成和分布的调节可能在呼吸道上皮细胞对臭氧暴露的反应中起重要作用。
Tenascin-C is an extracellular matrix component which is transiently expressed in association with epithelial cell detachment, proliferation, and migration. This molecule has been identified in respiratory tissue, but little is known about the cellular source of tenascin-C or the factors that regulate its production. Since air pollutants are known to disrupt epithelial integrity, we investigated the regulation of tenascin-C in response to 0.3 ppm ozone in differentiated primate nasal epithelial cells in culture at an air–medium interface. The expression of tenascin-C was upregulated in response to ozone, as determined by Northern blot analysis, Western blotting, and immunofluorescent staining. In contrast, there was no change in the mRNA levels for versican, biglycan, perlecan, or collagen type I. Reduced cellular attachment to the substrate was evident in ozone-treated cultures in association with tenascin-C deposition at the interfaces between cells and basal surfaces. The presence of tenascin-C on denuded areas of the matrix suggests that tenascin-C may have been instrumental in the loss of patches of cells. The modulation of tenascin-C synthesis and distribution may play a significant role in the response of respiratory epithelial cells to ozone exposure.