POSTBURN IMMUNE SUPPRESSION - AN INFLAMMATORY RESPONSE TO THE BURN WOUND
POSTBURN IMMUNE SUPPRESSION - AN INFLAMMATORY RESPONSE TO THE BURN WOUND
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DOI：
10.1097/00005373-199006000-00003
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发表时间：
1990-06-01
影响因子：
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通讯作者：
HOYT, D
中科院分区：
文献类型：
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作者：
HANSBROUGH, JF;ZAPATASIRVENT, R;HOYT, D
为了阐明严重烧伤后免疫抑制的原因，我们研究了烧伤皮肤皮下植入和其他材料植入对小鼠免疫功能的影响。移植后10d，培养3d后，检测脾淋巴细胞增殖反应和淋巴细胞表面活化抗原(IL-2R和Ia)的表达。此外，使用流式细胞术和一种与细胞内过氧化氢反应的染料分析了腹膜中性粒细胞的氧化爆发活性。植入2±2 cm的烧伤/未烧伤皮肤，以及植入类似大小的棉纱或胶原膜，随后导致淋巴细胞活化、增殖和中性粒细胞氧化爆发活性的抑制。对烧伤创面的强烈局部炎症反应可能导致严重烧伤后的全身免疫抑制。
In an effort to elucidate the causes of immune suppression which follows severe burn injury, we studied the immunologic effects of subcutaneous implantation of burned skin, as well as implantation of other materials, in mice. Ten days following the implantation, splenic lymphocyte proliferation and lymphocyte surface expression of activation antigens (IL-2R and Ia) were analyzed following a 3-day culture period. In addition, peritoneal neutrophils were analyzed for oxidative burst activity using flow cytometry and a dye which reacts with intracellular hydrogen peroxide. Implantation of a 2±2 cm section of burned/unburned skin as well as implantation of a similar-sized piece of cotton gauze or collagen sheet resulted in subsequent suppression of both lymphocyte activation proliferation and neutrophil oxidative burst activity. An intense local inflammatory response to the burn wound may play a role leading to the profound systemic immune suppression which follows severe burn injury.