Combined deficiency of iron and other divalent cations mitigates the symptoms of iron deficiency in tobacco plants
Combined deficiency of iron and other divalent cations mitigates the symptoms of iron deficiency in tobacco plants
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DOI：
10.1034/j.1399-3054.2003.00126.x
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发表时间：
2003-11-01
影响因子：
6.4
通讯作者：
Nishizawa, NK
中科院分区：
文献类型：
--
作者：
Kobayashi, T;Yoshihara, T;Nishizawa, NK
为了确定植物对多种金属缺乏的反应，对烟草(Nictiana tabacum L.)接受铁和另外两种微量营养素锌和锰的组合不足的处理。用植株外观、FW、叶绿素浓度和矿物质浓度等宏观指标和大麦(Hordeum uggare L.)IDS2启动子/GUS融合基因系统(Yoshihara等人2003，植物生物技术20：33-41)。在复合缺铁条件下生长的烟草植株比只缺铁的植株生长得更好，并具有更高的叶绿素浓度，尽管在植株组织中测得的铁浓度基本相同。当有铁存在时，Ids2/GUS的表达对缺铁有反应，但对缺锰或缺锌没有反应。在复合缺铁条件下生长的烟草植株有明显的GUS活性，但其反应显著低于只缺铁的烟草植株。缺铁症状在可见和分子水平上都得到了缓解。虽然需要更精确的实验证据来解释缓解机制，但矿物质的平衡被证明是根据烟草植株的反应来估计缺铁的一个重要参数。
To determine the responses of plants to deficiencies of multiple metals, tobacco plants (Nicotiana tabacum L.) were subjected to treatments that were deficient in combinations of Fe and two other micronutrients, Zn and Mn. The response was measured using macro indices, including plant appearance, FW, chlorophyll concentration, and mineral concentrations, and with a molecular index, the barley (Hordeum vulgare L.) Ids2 promoter/GUS fusion gene system (Yoshihara et al. 2003, Plant Biotech 20: 33-41). Tobacco plants grown in medium with combined deficiencies grew better and had higher chlorophyll concentrations than did plants grown on medium deficient in Fe only, although the measured Fe concentrations in the plant tissues were essentially the same. The Ids2/GUS expression responded to Fe deficiency, but not to Mn or Zn deficiencies in tobacco plants when Fe was present. Tobacco plants grown in medium with combined deficiencies had clearly detectable GUS activity, but the response was significantly lower than that in tobacco plants deficient in Fe only. The Fe-deficiency symptoms were mitigated at both the visible and molecular levels. Although more precise experimental evidence is needed to explain the mitigation mechanism, the balance of minerals was shown to be an important parameter to consider when estimating iron deficiency based on tobacco plant responses.