The hsp70 protein is involved in the acquisition of gamete self-sterility in the ascidian Ciona intestinalis.
The hsp70 protein is involved in the acquisition of gamete self-sterility in the ascidian Ciona intestinalis.
复制标题
hsp70 蛋白参与海鞘海鞘配子自不育的获得。
作者：
R. Marino;M. Pinto;F. Cotelli;C. L. Lamia;R. Santis
在两性海鞘中，配子自交不亲和是一种阻止自交受精的机制，其基础是卵母细胞的卵黄外壳区分和接受异种精子的能力。自体不孕发生在卵子发生过程中，涉及卵泡细胞或由卵泡细胞控制。配子自我-非我识别是一个可以比作免疫识别事件的过程，它代表了一个有用的模型，可以用来研究自我-非我识别的进化。HSP70基因属于主要组织相容性复合体(MHC)III类，被认为是MHC I类和II类基因的祖先，而伴侣蛋白参与了抗原的加工和提呈。我们已经分离并鉴定了一个HSP70基因(Cihsp70)，它在卵子发生前和卵黄发生前的卵泡细胞中结构性表达。使用针对Cihsp70蛋白的多克隆抗体，我们证明了Cihsp70的表达是从自育到自不育的转换所必需的。Cihsp70在配子自我非我识别中的功能参与为原脊索动物中存在类似MHC的祖先系统提供了证据。
In the hermaphrodite ascidian Ciona intestinalis, gamete self-incompatibility is a mechanism that prevents self-fertilization and is based on the ability of the oocyte vitelline coat to distinguish and accept only heterologous spermatozoa. The onset of self-sterility occurs during oogenesis and involves or is controlled by the follicle cells. Gamete self-nonself discrimination, a process that can be likened to an immune recognition event, represents a useful model with which to study the evolution of self-nonself recognition. Hsp70 genes, which belong to the major histocompatibility complex (MHC) class III, are supposedly ancestors of the MHC class I and II genes, and chaperonins are known to be involved in antigen processing and presentation. We have isolated and characterized an hsp70 gene (Cihsp70) that is constitutively expressed during oogenesis in the follicle cells of previtellogenic and vitellogenic oocytes. Using a polyclonal antibody against Cihsp70 protein, we demonstrate that the expression of Cihsp70 is required for the switch from self-fertility to self-sterility. The functional involvement of Cihsp70 in gamete self-nonself recognition provides evidence for an ancestral MHC-like system in protochordates.
影响因子：
56.9
作者：
SUTO, R;SRIVASTAVA, PK
通讯作者：
SRIVASTAVA, PK
细胞内蛋白质与折叠的主要组织相容性复合物 I 类分子的关联。
影响因子：
4.4
作者：
Morris,ChanteyR;Reber,AdrianJ;Petersen,JasonL;Vargas,ShannaE;Solheim,JoyceC
通讯作者：
Solheim,JoyceC
主要组织相容性复合体的进化和发育调节。
DOI：
10.1615/critrevimmunol.v15.i1.20
发表时间：
1995
期刊：
Critical reviews in immunology.
影响因子：
--
作者：
Salter-Cid,L;Flajnik,MF
通讯作者：
Flajnik,MF