Hyperoxia Alters Ultrastructure and Induces Apoptosis in Leukemia Cell Lines
Hyperoxia Alters Ultrastructure and Induces Apoptosis in Leukemia Cell Lines
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DOI：
10.3390/biom10020282
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发表时间：
2020-02-01
期刊：
影响因子：
5.5
通讯作者：
Balestra, Costantino
中科院分区：
文献类型：
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作者：
De Bels, David;Tillmans, Frauke;Balestra, Costantino
氧合条件对肿瘤的生长和进展至关重要。最近的数据表明，暴露在常压高氧中后，癌细胞的增殖减少。这些变化与分维有关。本研究的目的是研究高氧对白血病细胞株的凋亡和形态的影响。两种造血淋巴样癌细胞系(T淋巴母细胞系JURKAT和B淋巴系CCRF-SB)在常压高氧(FiO(2)和GT；60%，+/-18h)条件下进行检测，并与标准组(FiO(2)=21%)进行比较。我们用半胱氨酸天冬氨酸氨基转移酶-3法检测细胞凋亡。细胞形态采用细胞学观察、染色后显微照相、分维计算软件分析。我们的结果表明，细胞培养暴露于短暂的常压高氧诱导细胞凋亡(升高的caspase-3)以及细胞复杂性的显著和早熟改变，突出表现为两种细胞系的分维增加。这些特征与显微镜分析记录到的结构变化(固缩核和细胞凋亡)有关。这种形态的改变可能是由于多种分子机制和癌细胞中的重排，导致细胞周期抑制和细胞凋亡，如caspase-3活性所示。与B细胞相比，T细胞对高氧的抵抗力似乎更弱。
Oxygenation conditions are crucial for growth and tumor progression. Recent data suggests a decrease in cancer cell proliferation occurring after exposure to normobaric hyperoxia. Those changes are associated with fractal dimension. The purpose of this research was to study the impact of hyperoxia on apoptosis and morphology of leukemia cell lines. Two hematopoietic lymphoid cancer cell lines (a T-lymphoblastoid line, JURKAT and a B lymphoid line, CCRF-SB) were tested under conditions of normobaric hyperoxia (FiO(2) > 60%, +/- 18h) and compared to a standard group (FiO(2) = 21%). We tested for apoptosis using a caspase-3 assay. Cell morphology was evaluated by cytospin, microphotography after coloration, and analysis by a fractal dimension calculation software. Our results showed that exposure of cell cultures to transient normobaric hyperoxia induced apoptosis (elevated caspase-3) as well as significant and precocious modifications in cell complexity, as highlighted by increased fractal dimensions in both cell lines. These features are associated with changes in structure (pycnotic nucleus and apoptosis) recorded by microscopic analysis. Such morphological alterations could be due to several molecular mechanisms and rearrangements in the cancer cell, leading to cell cycle inhibition and apoptosis as shown by caspase-3 activity. T cells seem less resistant to hyperoxia than B cells.