Methylation of ASC/TMS1, a proapoptotic gene responsible for activating procaspase-1, in human colorectal cancer.
Methylation of ASC/TMS1, a proapoptotic gene responsible for activating procaspase-1, in human colorectal cancer.
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DOI：
10.1016/j.canlet.2003.08.027
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发表时间：
2003-12
期刊：
影响因子：
9.7
通讯作者：
Taro Yokoyama;J. Sagara;Xin Guan;J. Masumoto;M. Takeoka;Y. Komiyama;K. Miyata;K. Higuchi;S. Taniguchi
中科院分区：
文献类型：
--
作者：
Taro Yokoyama;J. Sagara;Xin Guan;J. Masumoto;M. Takeoka;Y. Komiyama;K. Miyata;K. Higuchi;S. Taniguchi
ASC/TMS 1是一种前半胱氨酸天冬氨酸蛋白酶-1（procaspase-1）的促凋亡激活剂，据报道在人乳腺癌中异常甲基化。我们发现，ASC甲基化的五个人结肠癌细胞系缺乏ASC蛋白表达。用5-氮杂-2 ′-脱氧胞苷对这些缺乏ASC的细胞系进行去甲基化处理，可部分恢复ASC表达。甲基化ASC也在10例结直肠癌组织中的6例中检测到。尽管用抗ASC单克隆抗体免疫染色几乎没有观察到肿瘤组织整个区域中ASC的明显下调，但在结肠直肠肿瘤细胞的少数群体中鉴定出ASC的完全抑制。本文将讨论ASC甲基化诱导的ASC抑制在结直肠癌中的生物学意义。
ASC/TMS1, a proapoptotic activator of procaspase-1, was reported to be aberrantly methylated in human breast cancer. We found that ASC was methylated in three of five human colon cancer cell lines lacking ASC protein expression. Demethylation treatment of these cell lines lacking ASC with 5-aza-2′-deoxycytidine partially restored ASC expression. Methylated ASC was also detected in six of ten colorectal cancer tissues. Although clear down-regulation of ASC in the whole region of a tumor tissue was hardly observed by immunostaining with anti-ASC mAb, complete suppression of ASC was identified in a minor population of the colorectal tumor cells. The biological significance of ASC methylation inducible ASC suppression in colorectal cancer will be discussed.