Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis
Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis
复制标题
强直性脊柱炎的综合 GC-MS 和 LC-MS 血浆代谢组学分析
DOI：
10.1039/b807369d
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发表时间：
2008-09-01
期刊：
影响因子：
4.2
通讯作者：
Xu, Guowang
中科院分区：
文献类型：
--
作者：
Gao, Peng;Lu, Chen;Xu, Guowang
强直性脊柱炎（AS）是一种慢性炎症性关节炎，主要影响青少年患者的中轴骨骼。该疾病的自然史仍然知之甚少。在本研究中，我们结合GC-MS和LC-MS技术，从代谢组学的角度评估AS患者血浆中的主要代谢变化。采用单变量和多变量分析来进行改变的代谢物比较和模式识别。对 GC-MS 或 LC-MS 数据应用监督偏最小二乘判别分析可以准确地区分 AS 患者与正常对照，这证明了其潜在的诊断用途。此外，AS患者的脯氨酸、葡萄糖、磷酸盐、尿素、甘油、苯丙氨酸和同型半胱氨酸的血浆浓度升高，但磷酸胆碱、色氨酸和二肽——苯丙氨酰-苯丙氨酸的水平降低。在其涉及的代谢途径的背景下，对已鉴定的代谢物进行了相应的讨论。这项研究主要证明，集成的色谱-质谱分析和集成的单变量和多变量统计分析有助于代谢组学成为疾病研究中更有前途的工具。
Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that predominantly affects the axial skeleton in adolescent patients. The natural history of the disease remains poorly characterized. In this study, we combined GC-MS and LC-MS techniques to evaluate the major metabolic changes in the plasma of AS patients in view of metabonomics. Univariate and multivariate analysis were employed for altered metabolite comparison and pattern recognition. Application of supervised partial least-squares discrminant analysis to either GC-MS or LC-MS data allowed accurate discrimination of AS patients from normal controls, demonstrating its potential diagnostic utilization. In addition, AS patients presented elevated plasma concentrations of proline, glucose, phosphate, urea, glycerol, phenylalanine and homocysteine but reduced levels of phosphocholines, tryptophan and a bipeptide - phenylalanyl-phenylalanine. In the context of their involved metabolic pathways, the identified metabolites were discussed accordingly. This investigation primarily proved that integrated chromatography-mass spectrometry and integrated uni- and multi-variate statistical analysis facilitated metabonomics to be a more promising tool in disease research.