OVERPRODUCTION OF FTSZ INDUCES MINICELL FORMATION IN ESCHERICHIA-COLI
OVERPRODUCTION OF FTSZ INDUCES MINICELL FORMATION IN ESCHERICHIA-COLI
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DOI：
10.1016/0092-8674(85)90290-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
LUTKENHAUS, J
中科院分区：
文献类型：
--
作者：
WARD, JE;LUTKENHAUS, J
大肠杆菌 K-12 中的 ftsZ 基因是细胞分裂必需的基因。我们报告称，FtsZ 蛋白水平增加两到七倍会导致小细胞表型的诱导。 FtsZ 水平增加超过该范围会导致所有细胞分裂受到抑制。与经典的小细胞突变体不同，FtsZ水平增加诱导的小细胞的形成并不以正常分裂为代价，这表明FtsZ水平的增加导致每个细胞周期发生额外的分裂事件。此外，FtsZ 的增加导致细胞分裂在细胞周期的早期开始。这些结果与控制大肠杆菌细胞分裂频率的 FtsZ 水平或活性一致。
The ftsZ gene in E. coli K-12 is an essential cell division gene. We report that a two to sevenfold increase in the level of the FtsZ protein resulted in induction of the minicell phenotype. An increase in the level of FtsZ beyond this range resulted in an inhibition of all cell division. Unlike the classical minicell mutant, the formation of minicells induced by increased levels of FtsZ did not occur at the expense of normal divisions, indicating that increasing FtsZ resulted in additional division events per cell cycle. In addition, increased FtsZ caused cell division to be initiated earlier in the cell cycle. These results are consistent with the level or activity of FtsZ controlling the frequency of cell division in E. coli.