Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration
Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration
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DOI：
10.1067/mje.2002.117290
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发表时间：
2002-05-01
影响因子：
6.5
通讯作者：
Kaul, S
中科院分区：
文献类型：
--
作者：
Lindner, JR;Song, J;Kaul, S
第二代微泡超声造影剂的微血管流变学和肺部滞留程度以前没有得到很好的表征。我们评估的微血管行为的Depletion，一个脂质壳的微泡剂含有全氟丙烷气体，使用活体显微镜无论是大鼠脊髓背角肌或小鼠提睾肌。在动脉内注射后立即进行肺滞留建模，较大的微泡（> 5 μ m）被截留在小动脉和毛细血管内。微泡的保留分数较低（1.2% +/- 0.1%），截留是短暂的（85%在10分钟内脱落），未导致不良血流动力学影响。包埋部位未见白细胞或血小板粘附。静脉注射后，未观察到微泡截留，微泡在小动脉、小静脉和毛细血管中的速度与红细胞的速度具有良好的相关性。我们的结论是，静脉注射和肺通道后，去甲肾上腺素微泡的微血管流变学类似于红细胞。静脉注射后肺微循环内的微泡截留应可忽略不计且为一过性。这些发现对于确定该药物的安全性非常重要。
The microvascular rheology and extent of pulmonary retention of second-generation microbubble ultrasound contrast agents has not previously been well characterized. We assessed the microvascular behavior of Definity, a lipid-shelled microbubble agent containing perfluoropropane gas, using intravital microscopy of either rat spinotrapezius muscle or mouse cremaster muscle. Immediately after intra-arterial injection, which was performed to model pulmonary retention, larger microbubbles (> 5 mum) were entrapped within small arterioles and capillaries. The retention fraction of microbubbles was low (1.2% +/- 0.1%) and entrapment was transient (85% dislodged by 10 minutes), resulting in no adverse hemodynamic effects. Leukocyte or platelet adhesion at the site of entrapment was not seen. After intravenous injection, no microbubble entrapment was observed and the velocities of microbubbles in arterioles, venules, and capillaries correlated well with those of red blood cells. We conclude that after intravenous injection and pulmonary passage, the microvascular rheology of Definity microbubbles is similar to that of red blood cells. Microbubble entrapment within the pulmonary microcirculation after venous injection should be negligible and transient. These findings are important for establishing the safety of this agent.