Depolarized cell growth precedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis.
Depolarized cell growth precedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis.
复制标题
在热带假丝酵母中乙醇诱导的假菌丝形成过程中，去极化细胞生长先于丝状形成。
DOI：
10.1099/00221287-144-2-403
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发表时间：
1998
期刊：
影响因子：
1.5
通讯作者：
T. Kamihara
中科院分区：
文献类型：
--
作者：
T. Suzuki;Y. Imanishi;S. Iwaguchi;T. Kamihara
据报道，乙醇引起热带假丝酵母Pk233中的菌丝生长，并且还显示伴随加入肌醇可以消除菌丝生长。在这项研究中，乙醇诱导的菌丝生长在这种生物体的过程中，结合肌动蛋白定位的细胞学特征进行了研究。用乙醇培养得到双相生长曲线。在第一个生长阶段（倍增时间2.4小时），有一个肿胀的球形酵母细胞的积累，而不是在对照培养中观察到的椭圆形，随后出现的球形子细胞链。在这些肿胀的酵母细胞上观察到随机分布的肌动蛋白斑块，并且这些细胞的芽起始位点呈现随机性。这些观察结果表明，乙醇引起的细胞生长在第一阶段的去极化。在第二个生长阶段（倍增时间7.4小时），出现假菌丝细胞，从肿胀的酵母细胞伸出。对照培养物中几丁质酶活性在对数生长期上升。在乙醇培养物中，活性在整个生长阶段保持在低水平。当假菌丝细胞转移到新鲜的乙醇培养基中时，酵母细胞从假菌丝丝中出现，并改变其形状为球形，并且在第一阶段出现抑制。从这些观察结果，乙醇对C。tropicalis细胞出现细胞生长的去极化，并且由此产生的肿胀细胞生长为极性假菌丝细胞。在补充有乙醇和肌醇两者或乙醇和山梨醇两者的培养物中，未观察到肿胀细胞的积累，并且在整个生长阶段中观察到具有正常椭圆形形状的单个酵母细胞。
Ethanol has been reported to cause mycelial growth in Candida tropicalis Pk233, and mycelial growth has also been shown to be abolished by concomitant addition of myo-inositol. In this study, the process of ethanol-induced mycelial growth in this organism was examined in combination with cytological characterization of actin localization. Cultivation with ethanol gave biphasic growth curves. During the first growth phase (doubling time 2.4 h), there was an accumulation of swollen spherical yeast cells, instead of the oblong ones observed in the control culture, followed by the appearance of spherical daughter cells in chains. Randomly-distributed actin patches were observed on these swollen yeast cells and the bud initiation sites of these cells appeared random. These observations suggested that ethanol caused depolarization of cell growth during the first phase. During the second growth phase (doubling time 7.4 h), pseudohyphal cells appeared, projecting from the swollen yeast cells. Activity of chitinase in the control culture rose during the exponential phase. In the ethanol culture the activity stayed at a low level throughout the growth phases. When pseudohyphal cells were transferred to fresh ethanol medium, yeast cells appeared from pseudohyphal filaments and changed their shape to spherical, and filamentation appeared to be inhibited during the first phase. From these observations, an initial effect of ethanol on C. tropicalis cells appeared to be depolarization of cell growth, and the resulting swollen cells grew as polar pseudohyphal cells. In the culture supplemented with both ethanol and inositol, or with both ethanol and sorbitol, the accumulation of swollen cells was not observed and single yeast cells with normal oblong shape were seen throughout the growth phases.
DOI：
10.1016/s0021-9258(18)55057-2
发表时间：
1991-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Kuranda;P. Robbins
通讯作者：
M. Kuranda;P. Robbins
影响因子：
56.9
作者：
LIU, HP;STYLES, CA;FINK, GR
通讯作者：
FINK, GR