Functional computer-to-plate near-infrared absorbers as highly efficient photoacoustic dyes
Functional computer-to-plate near-infrared absorbers as highly efficient photoacoustic dyes
复制标题
作为高效光声染料的功能性计算机直接制版近红外吸收剂
DOI：
10.1016/j.actbio.2016.07.026
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发表时间：
2016
影响因子：
9.7
通讯作者：
Chen Xuesi
中科院分区：
文献类型：
--
作者：
Niu Yuewei;Song Wantong;Zhang Dawei;Tang Zhaohui;Deng Mingxiao;Chen Xuesi
光声成像(PAI)是一种新兴的生物医学成像技术。光声效应是PAI的基础，光声造影剂吸收光脉冲来启动局部加热和快速热膨胀，从而产生热弹性应力波。因此，理想的PAI染料应具有较强的近红外光吸收能力和较高的光热转换效率。然而，目前大多数低分子有机PAI造影剂都是荧光染料，由于荧光发射的能量损失，导致光热转换效率大大降低。在此，我们报道了一系列具有单键COOH、单键NH_2和NHS酯官能团的高效光声染料，来自廉价的工业计算机对板近红外吸收剂(IR830P-甲苯磺酸盐)，它具有很强的近红外吸收能力，但荧光发射极低。体内外研究表明，IR830染料具有低细胞毒性，在光声成像中比传统的光声染料吲哚青绿(ICG)亮度高2.1倍。IR830系列染料的最低定量下限为ICG的1/7。这表明IR830功能染料作为高效光声染料具有很大的潜力。光声成像(PAI)是一种新兴的生物医学成像手段。理想的PAI染料应具有较强的近红外吸收能力和较高的光热转换效率。然而，目前大多数低分子有机PAI造影剂都是荧光染料，由于荧光发射的能量损失，导致光热转换效率大大降低。在这里，我们报道了一系列高效的功能光声染料，它来自廉价的工业计算机-平板近红外吸收材料(IR830)，它具有很强的近红外吸收能力，但荧光发射极低。功能染料IR830具有较低的细胞毒性，在光声成像中的亮度远高于传统的光声染料吲哚青绿。这些结果表明，功能染料IR830作为高效光声染料具有很大的潜力。
Photoacoustic imaging (PAI) is an emerging modality in biomedical imaging. Photoacoustic effect is the basis for PAI, where a photoacoustic contrast agent absorbs optical pulses to initiate localized heating and rapid thermal expansion, thus generating thermoelastic stress waves. Therefore, ideal PAI dyes should have strong NIR light absorbance and high light-heat conversion efficiency. However, most current low molecular weight organic PAI contrast agents are fluorescent dyes, where the light-heat conversion efficiency is dramatically impaired due to the energy loss by fluorescence emission. Herein, we report a series of highly efficient photoacoustic dyes with single bondCOOH, single bondNH2and NHS ester functionalities, from an inexpensive industrial computer-to-plate NIR absorber (IR830p-toluenesulfonate) that has a strong NIR absorbance but an extremely low fluorescence emission.In vitroandin vivostudies show that the functional IR830 dyes have low cytotoxicity, and are 2.1 folds brighter in photoacoustic imaging than traditional photoacoustic dye indocyanine green (ICG). The Lowest Limit of Quantification of the IR830 series dyes is as low as the 1/7 of that of ICG. These indicate that the functional IR830 dyes have great potential as highly efficient photoacoustic dyes.Statement of SignificancePhotoacoustic imaging (PAI) is an emerging modality in biomedical imaging. Ideal PAI dyes should have strong NIR absorbance and high light-heat conversion efficiency. However, most current low molecular weight organic PAI contrast agents are fluorescent dyes, where the light-heat conversion efficiency is dramatically impaired due to the energy loss by fluorescence emission. Herein we report a series of highly efficient functional photoacoustic dyes from an inexpensive industrial computer-to-plate NIR absorber (IR830) that has a strong NIR absorbance but an extremely low fluorescence emission. The functional IR830 dyes show low cytotoxicity, much brighter in photoacoustic imaging than traditional photoacoustic dye indocyanine green. These indicate that the functional IR830 dyes have great potential as highly efficient photoacoustic dyes.