Cytokine-mediated PGE2 expression in human colonic fibroblasts
Cytokine-mediated PGE2 expression in human colonic fibroblasts
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DOI：
10.1152/ajpcell.1998.275.4.c988
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发表时间：
1998-10-01
影响因子：
5.5
通讯作者：
Lance, P
中科院分区：
文献类型：
--
作者：
Kim, EC;Zhu, YT;Lance, P
我们研究了人结肠成纤维细胞（CCD-18Co 和 5 个原代成纤维细胞培养物）和上皮细胞系（NCM460、T84、HT-29 和 LS 174T）中的前列腺素生物合成以及 PGE(2) 对成纤维细胞形态的影响。测量细胞因子刺激的PGE(2) 产生。评估了PGH合酶-1和-2（PGHS-1和-2）蛋白和mRNA表达。所有细胞类型的基础 PGE(2) 水平均较低（0.15-6.47 ng/mg 蛋白质）。分别用白细胞介素-1β（IL-1β；10ng/ml）或肿瘤坏死因子-α（50ng/ml）处理24小时，在CCD-18Co培养物中诱导PGE（2）合成最大25倍和6倍，在原代成纤维细胞培养物中得到类似结果；在结肠上皮细胞系中IL-1β的最大诱导是零到五倍。用IL-1β处理CCD-18Co成纤维细胞导致PGHS-2蛋白和mRNA水平分别最大增加21倍和53倍，而不改变PGHS-1表达。 PGE(2) (0.1 mu mol/l) 在选定的成纤维细胞中引起显着的形状变化。结肠成纤维细胞作为细胞因子靶标以及体内结肠前列腺素的来源和靶标具有潜在的重要作用。
We investigated prostanoid biogenesis in human colonic fibroblasts (CCD-18Co and 5 primary fibroblast cultures) and epithelial cell lines (NCM460, T84, HT-29, and LS 174T) and the effect of PGE(2) on fibroblast morphology. Cytokine-stimulated PGE(2) production was measured. PGH synthase-1 and -2 (PGHS-1 and -2) protein and mRNA expression were evaluated. Basal PGE(2) levels were low in all cell types (0.15-6.47 ng/mg protein). Treatment for 24 h with interleukin-1 beta (IL-1 beta; 10 ng/ml) or tumor necrosis factor-alpha (50 ng/ml), respectively, elicited maximal 25- and 6-fold inductions of PGE(2) synthesis in CCD-18Co cultures and similar results in primary fibroblast cultures; maximal inductions with IL-1 beta in colonic epithelial cell lines were from zero to fivefold. Treatment of CCD-18Co fibroblasts with IL-1 beta caused maximal 21- and 53-fold increases, respectively, in PGHS-2 protein and mRNA levels without altering PGHS-1 expression. PGE(2) (0.1 mu mol/l) elicited a dramatic shape change in selected fibroblasts. Colonic fibroblasts are potentially important as cytokine targets and a source of and target for colonic prostanoids in vivo.