Cryo-electron microscopy of vitrified insect flight muscle.
Cryo-electron microscopy of vitrified insect flight muscle.
复制标题
玻璃化昆虫飞行肌的冷冻电子显微镜。
DOI：
10.1016/0022-2836(84)90233-x
复制
发表时间：
1984
影响因子：
5.6
通讯作者：
J. Dubochet
中科院分区：
文献类型：
--
作者：
A. McDowall;W. Hofmann;J. Lepault;M. Adrian;J. Dubochet
用冷冻电镜研究了昆虫飞行肌在僵直和松弛状态下的玻璃化冷冻水化切片。获得了具有良好对比度的高分辨率图像。从直接图像和光学衍射图与从甘油化的肌纤维获得的X射线衍射图的比较来判断，玻璃化的标本保存良好。避免了传统塑料切片中遇到的制备伪影。松弛结构的保存似乎也比冷冻蚀刻的复制品更好，因为在厚细丝上的14.5 nm重复的横桥很容易看到。
Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy. High resolution images with good contrast were obtained. As judged from direct images and from the comparison of optical diffractograms with X-ray diffraction patterns obtained from glycerinated muscle fibres, the vitrified specimen is well preserved. Preparation artifacts encountered in conventional plastic sections are avoided. The preservation of the relaxed structure also seems better than in freeze-etched replicas as the 14.5 nm repeat of crossbridges on thick filaments is easily visible.