Methods for culturing human embryonic stem cells in a xeno-free system.
Methods for culturing human embryonic stem cells in a xeno-free system.
复制标题
DOI：
10.1007/978-1-62703-348-0_9
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Boucher, Shayne
中科院分区：
文献类型：
--
作者：
Kaur, Jasmeet;Tilkins, Mary Lynn;Eckert, Richard;Boucher, Shayne
Defined pluripotent stem cell culture media is a valuable tool for tracking and analyzing morphological, cell signaling and gene expression changes in human embryonic stem cells. Cultivation of hESC under xeno-free culture settings provides researchers with a consistent and reproducible environment to test experimental hypotheses and move towards translational and clinical research. One of the primary concerns of the xenogeneic culture is the transfer of foreign pathogens or antigens that induce disease or immune response by the patient. These undesirable by-products may come from the use of murine-derived feeder cells, xenogeneic matrices, or from animal-derived components found in the cell culture medium or matrix used to isolate or expand the stem cells. This chapter describes standardized protocols for obtaining consistent and reproducible results for culturing PSC under xeno-free, feeder-free, or feeder-based systems.
