An analysis of the immunological tumor microenvironment of primary tumors and regional lymph nodes in squamous cell lung cancer.
An analysis of the immunological tumor microenvironment of primary tumors and regional lymph nodes in squamous cell lung cancer.
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鳞状细胞肺癌原发肿瘤及区域淋巴结的免疫肿瘤微环境分析。
DOI：
10.21037/tlcr-21-479
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发表时间：
2021-08
影响因子：
4
通讯作者：
Ishida T
中科院分区：
文献类型：
--
作者：
Ichiki Y;Ueno M;Yanagi S;Kanasaki Y;Goto H;Fukuyama T;Mikami S;Nakanishi K;Ishida T
在抗肿瘤免疫中发挥核心作用的各种免疫细胞在原发性肿瘤和局部淋巴结中积累。分析这种细胞积累和分子表达以阐明免疫肿瘤微环境。纳入50例肺鳞癌患者，均行完全切除术。对手术切除的原发性肺肿瘤和区域淋巴结标本进行免疫组化染色，检测CD 8、CD 103、主要组织相容性复合物（MHC）I类和程序性细胞死亡蛋白配体1（PD-L1）等免疫相关分子，并回顾性分析其预后与临床病理因素的关系。单因素分析显示，肿瘤浸润淋巴细胞和CD 8+淋巴细胞、肿瘤内和间质内CD 103+淋巴细胞、肿瘤直径、病理T和N因子以及病理分期是影响疾病特异性生存期（DSS）的重要预后因素。在多因素分析中，肿瘤内和间质内CD 103+淋巴细胞和病理T和N因子是DSS的独立预后因素。原发肿瘤和转移淋巴结的PD-L1表达之间以及肿瘤浸润淋巴细胞、CD 8+淋巴细胞和CD 103+淋巴细胞之间存在显著一致性。CD 103+淋巴细胞浸润到肿瘤中与原发性肿瘤和区域淋巴结转移中癌细胞的PD-L1表达增加显著相关。淋巴结转移瘤内CD 103+淋巴细胞浸润和癌细胞PD-L1表达均显著高于原发性肿瘤。原发肿瘤中CD 103+淋巴细胞浸润与预后密切相关。
Various immune cells that play a central role in antitumor immunity accumulate in primary tumors and regional lymph nodes. Such cellular accumulation and the molecular expression were analyzed to elucidate the immunological tumor microenvironment. Fifty squamous cell lung cancer patients with complete resection were included. Resected specimens from primary lung tumors and regional lymph nodes were immunostained for immune-related molecules, such as CD8, CD103, major histocompatibility complex (MHC) class I, and programmed cell death protein ligand-1 (PD-L1), and the relationship between the prognosis and clinicopathological factors was retrospectively analyzed. Tumor-infiltrating lymphocytes and CD8+ lymphocytes, intratumoral and intrastromal CD103+ lymphocytes, tumor diameter, pathological T and N factors, and pathological stage were significant prognostic factors for the disease-specific survival (DSS) in a univariate analysis. In a multivariate analysis, intratumoral and intrastromal CD103+ lymphocytes and pathological T and N factors were independent prognostic factors of the DSS. Significant concordance was found between the PD-L1 expression of primary tumors and metastatic lymph nodes as well as among tumor-infiltrating lymphocytes, CD8+ lymphocytes and CD103+ lymphocytes. Infiltration of CD103+ lymphocytes into the tumor was significantly correlated with an increased PD-L1 expression of cancer cells in both primary tumors and reginal lymph node metastases. Both the intratumoral infiltration of CD103+ lymphocytes and PD-L1 expression of cancer cells were significantly higher in lymph node metastases than in primary tumors. CD103+ lymphocyte infiltration in the primary tumor was shown to be strongly involved in the prognosis.