Diacylglycerol kinase zeta is involved in the process of cerebral infarction.
Diacylglycerol kinase zeta is involved in the process of cerebral infarction.
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发表时间：
2006
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通讯作者：
T. Nakano;Y. Hozumi;H. Ali;Sachiko Saino-Saito;H. Kamii;Shinya Sato;T. Kayama;Masahiko Watanabe;H. Kondo;K. Goto
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作者：
T. Nakano;Y. Hozumi;H. Ali;Sachiko Saino-Saito;H. Kamii;Shinya Sato;T. Kayama;Masahiko Watanabe;H. Kondo;K. Goto
甘油二酯激酶（DGK）是一种磷酸化第二信使甘油二酯（DG）的酶，参与多种病理生理细胞反应。我们以前曾报道DGKzeta可能参与短暂性前脑缺血时海马CA 1区神经元的选择性易损性。在这项研究中，我们的目的是进一步阐明DGK同工酶的功能意义，在大脑皮层梗死使用局灶性缺血模型。在大脑中动脉闭塞90分钟的早期阶段，DGKzeta免疫反应性在缺血核心的皮质神经元的核中迅速降低，而DGKzeta和其他神经元蛋白如MAP-2和NeuN保持完整。这表明缺血神经元中DGKzeta的快速消失是响应于缺血的神经元变性的相当早期的事件。此外，在梗死的晚期炎症阶段，在非神经元细胞中检测到DGKzeta免疫反应性，包括因子VIII阳性内皮细胞和艾德-1阳性吞噬细胞。本研究提示DGKzeta可能在缺血性脑损伤的多种过程中发挥作用，包括神经元死亡和非神经元炎症反应。
Diacylglycerol kinase (DGK) is an enzyme that phosphorylates a second messenger diacylglycerol (DG) and is involved in a variety of pathophysiological cellular responses. We have previously reported that DGKzeta may be involved in the selective vulnerability of hippocampal CA1 neurons in transient forebrain ischemia. In this study we aimed to further elucidate functional implications of DGK isozymes in the cerebral cortex suffering from infarction using a focal ischemic model. In the early phase of 90 min of middle cerebral artery occlusion, DGKzeta-immunoreactivity is reduced rapidly in the nucleus of cortical neurons in the ischemic core, while DGKiota and other neuronal proteins such as MAP-2 and NeuN remain intact. This suggests that rapid disappearance of DGKzeta in ischemic neurons is a quite early event precedent to neuronal degeneration in response to ischemia. Furthermore, in the late inflammatory phase of infarction DGKzeta-immunoreactivity is detected in non-neuronal cells including factor VIII-positive endothelial cells and ED-1-positive phagocytic cells. The present study suggests that DGKzeta may play roles in various processes of ischemic brain damage including neuronal death and non-neuronal inflammatory response.