The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
复制标题
在对间期核和中期染色体进行核酸酶处理后，仍与支架结合的 DNA 序列的相似性。
作者：
S. Razin;V. L. Mantieva;G. Georgiev
获得了附着在间期细胞核或中期染色体蛋白骨架上的DNA片段。使用了限制性内切酶处理/1,2/和基于轻度葡萄球菌核酸酶消化的新方法。在后一种情况下，留在细胞核或染色体上的DNA片段在卫星中不富集，而只在大量的中间重复DNA中富集。附着DNA片段越短，片段中中间重复DNA的含量越高。与主DNA相比，它在CsCl梯度中的密度略高。间期和中期染色体的附着DNA的产量、密度梯度分布和恢复特性基本相同。中期染色体和间期细胞核的DNA环平均大小约为60kb。结果表明，DNP原纤维与染色体轴向结构的大部分附着位点在细胞周期中保持不变。
The fragments of DNA attached to protein skeleton of interphase nuclei or metaphase chromosomes were obtained. Both the method involving restriction endonuclease treatment/1,2/and a novel procedure based on mild staphylococcal nuclease digestion were used. In the latter case, DNA fragments remaining bound to nuclei or chromosomes are not enriched in satellite but only in abundant middle repetitive DNA. The shorter the fragments of attached DNA, the higher the content of middle repetitive DNA in the fraction. It has a slightly higher density in a CsCl gradient comparing to the main DNA. The yield of attached DNA, its distribution in a CsCl density gradient, and its renaturation properties are essentially the same for interphase and metaphase chromosomes. The average size of DNA loops was found to be equal to approximately 60 kb for both metaphase chromosomes and interphase nuclei. The conclusion has been drawn that the bulk of attachment sites of DNP fibrils to axial chromosomal structures remains unchanged during the cell cycle.