Quantitative Confocal Imaging of the Retinal Microvasculature in the Human Retina
Quantitative Confocal Imaging of the Retinal Microvasculature in the Human Retina
复制标题
DOI：
10.1167/iovs.12-10017
复制
发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Yu, Dao-Yi
中科院分区：
文献类型：
--
作者：
Tan, Priscilla Ern Zhi;Yu, Paula K.;Yu, Dao-Yi
目的.我们定量研究了人类视网膜不同神经层中血管的分布。方法。总共16只人类供体眼睛进行灌注固定并标记内皮f-肌动蛋白。视网膜偏心位于3毫米以上的视盘上级进行了研究，使用共聚焦扫描激光显微镜。免疫组织化学方法应用于整体安装和横截面共定位毛细血管网络与神经元元素。毛细血管形态，直径和密度测量进行了比较网络。在以下区域中识别并定量了四种不同的毛细血管网络：神经纤维层（NFL）、视网膜神经节细胞（RGC）层、内丛状层（IPL）的边界和内核层（INL）的表面边界以及深INL和外丛状层的边界。最里面和最外面的毛细血管网络表现出层状配置，而IPL和深INL网络显示出复杂的三维配置。RGC和IPL网络中的毛细血管直径显著小于其他网络。毛细血管密度在RGC网络中最大（26.74%），并且显著大于NFL（13.69%）、IPL（11.28%）和深INL（16.12%）网络。神经元亚室的独特代谢需求可能会影响区域毛细血管网络的形态特征。毛细血管直径和密度之间的网络的差异可能有重要的相关性，在人类视网膜的神经元功能。这些发现可能对了解视网膜血管疾病的致病机制很重要。（Invest Ophthalmol维斯科学。2012;53：5728-5736）DOI：10.1167/iovs.12-10017
PURPOSE. We investigated quantitatively the distribution of blood vessels in different neural layers of the human retina.METHODS. A total of 16 human donor eyes was perfusion-fixed and labeled for endothelial f-actin. Retinal eccentricity located 3 mm superior to the optic disk was studied using confocal scanning laser microscopy. Immunohistochemical methods applied to whole-mount and transverse sections were used to colocalize capillary networks with neuronal elements. Capillary morphometry, diameter, and density measurements were compared among networks.RESULTS. Four different capillary networks were identified and quantified in the following regions: Nerve fiber layer (NFL), retinal ganglion cell (RGC) layer, border of the inner plexiform layer (IPL) and superficial boundary of the inner nuclear layer (INL), and boundary of the deep INL and outer plexiform layer. The innermost and outermost capillary networks demonstrated a laminar configuration, while IPL and deep INL networks displayed a complex three-dimensional configuration. Capillary diameter in RGC and IPL networks were significantly less than in other networks. Capillary density was greatest in the RGC network (26.74%), and was significantly greater than in the NFL (13.69%), IPL (11.28%), and deep INL (16.12%) networks.CONCLUSIONS. The unique metabolic demands of neuronal sub-compartments may influence the morphometric features of regional capillary networks. Differences in capillary diameter and density between networks may have important correlations with neuronal function in the human retina. These findings may be important for understanding pathogenic mechanisms in retinal vascular disease. (Invest Ophthalmol Vis Sci. 2012;53:5728-5736) DOI: 10.1167/iovs.12-10017