Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and Timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively.
Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and Timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively.
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DOI：
10.1006/geno.1998.5362
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发表时间：
1998-07
期刊：
影响因子：
4.4
通讯作者：
T. Olson;S. Hirohata;J. Ye;K. Leco;M. F. Seldin;S. Apte
中科院分区：
文献类型：
--
作者：
T. Olson;S. Hirohata;J. Ye;K. Leco;M. F. Seldin;S. Apte
我们已经分离出含有人类金属蛋白酶组织抑制剂-4基因（TIMP-4）的基因组DNA，并确定了该基因的外显子结构。与TIMP家族的其他成员一样，TIMP-4蛋白由五个外显子编码。它们跨越6 kb的基因组DNA，因此TIMP 4的大小与TIMP 1相似，但比TIMP 2和TIMP 3小得多。TIMP 4的外显子-内含子边界与其他TIMP基因的位置非常相似，表明该家族中基因结构的高度保守性。人类和小鼠TIMP-4基因定位于各自基因组中的可比位置，定位于人类染色体3 p25和小鼠染色体6。
We have isolated genomic DNA containing the human tissue inhibitor of metalloproteinases-4 gene (TIMP4) and determined the structure of the exons comprising the gene. Like other members of the TIMP family, the TIMP-4 protein is encoded by five exons. These span 6 kb of genomic DNA, so that TIMP4 is similar in size to Timp1 but considerably smaller than TIMP2 and TIMP3. The exon-intron boundaries of TIMP4 are at locations very similar to those of the other TIMP genes, demonstrating the high degree of conservation of gene structure in this family. The human and mouse TIMP-4 genes map to comparable locations in the respective genomes, localizing to human chromosome 3p25 and mouse chromosome 6.