A high-throughput platform for interpretation of metabolite profile data from pepper (Capsicum) fruits of 13 phenotypes associated with different fruit maturity states
A high-throughput platform for interpretation of metabolite profile data from pepper (Capsicum) fruits of 13 phenotypes associated with different fruit maturity states
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DOI：
10.1016/j.foodchem.2020.127286
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发表时间：
2020-11-30
期刊：
影响因子：
8.8
通讯作者：
Kim, Jae Kwang
中科院分区：
文献类型：
--
作者：
Kim, Tae Jin;Hyeon, Hyejin;Kim, Jae Kwang
如今，新的工具已被开发用于与代谢途径相关的大规模代谢物谱数据的有效分析和可视化。首次提出了一个使用PathPathPath 3结合多变量分析的高通量平台。首次分析了辣椒果实成熟过程中萜类化合物单萜、倍半萜、三萜和四萜之间的相互关系及其变化。该平台已成功应用于解释13个辣椒表型的成熟和未成熟果实中131种代谢产物的大规模数据。辣椒果实中类胡萝卜素衍生的挥发性物质如二氢猕猴桃内酯和β-紫罗兰酮与类胡萝卜素的含量密切相关，表明辣椒果实成熟期类胡萝卜素的合成和降解发生在果实成熟期。利用Pathology 3可以快速呈现途径中的代谢变化，揭示成熟期脯氨酸、辣椒素、苯丙氨酸等逆境相关代谢产物的积累。该方法可为果实成熟的综合生化调控提供有用的信息。
Nowadays, novel tools have been developed for efficient analysis and visualization of large-scale metabolite profile data associated with metabolic pathways. A high-throughput platform using PathVisio 3 combined with multivariate analysis is proposed for the first time. Additionally, this is the first analysis of the relationships among terpenoids monoterpene, sesquiterpene, triterpene, and tetraterpene during pepper fruit ripening, and their changes. This platform was successfully applied to interpret large-scale data related to 131 metabolites from mature and immature fruits of 13 pepper phenotypes. The carotenoid-derived volatiles, such as dihydroactinidiolide and beta-ionone were closely correlated with carotenoids, indicating that the synthesis and degradation of carotenoids occurred in pepper fruit mature stage. Using PathVisio 3, the metabolic changes in pathway could be presented quickly, revealing the accumulation of stress-related metabolites, such as proline, capsaicin, and phenylalanine, in the mature stage. This approach could provide useful information about comprehensive biochemical regulation of fruit ripening.