SABRE populates ER domains essential for cell plate maturation and cell expansion influencing cell and tissue patterning.
SABRE populates ER domains essential for cell plate maturation and cell expansion influencing cell and tissue patterning.
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DOI：
10.7554/elife.65166
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发表时间：
2021-03-09
期刊：
影响因子：
7.7
通讯作者：
Bezanilla M
中科院分区：
文献类型：
--
作者：
Cheng X;Bezanilla M
SABRE在整个真核生物中发现，最初在植物中鉴定，介导植物中的细胞扩增、分裂平面取向和平面极性。SABRE如何以及在哪里调解这些过程仍然是一个悬而未决的问题。我们删除了立碗藓中的SABRE，立碗藓是细胞生物学的优秀模型。SABRE无效突变体发育不良，与种子植物中的表型相似。此外，极化生长的细胞在胞质分裂中被延迟，有时导致灾难性的失败。一种功能性SABRE荧光融合蛋白，在间期定位于内质网（ER）区域上的动态斑点，在细胞分裂期间定位于细胞板上。没有SABRE，细胞积累ER聚集体，并且ER沿着发育中的细胞板沿着异常弯曲。值得注意的是，胼胝质沉积延迟，并在细胞分裂失败，异常胼胝质积累形成在细胞板。我们的研究结果揭示了ER在细胞板成熟中的令人惊讶的和基本的作用。
SABRE, which is found throughout eukaryotes and was originally identified in plants, mediates cell expansion, division plane orientation, and planar polarity in plants. How and where SABRE mediates these processes remain open questions. We deleted SABRE in Physcomitrium patens, an excellent model for cell biology. SABRE null mutants were stunted, similar to phenotypes in seed plants. Additionally, polarized growing cells were delayed in cytokinesis, sometimes resulting in catastrophic failures. A functional SABRE fluorescent fusion protein localized to dynamic puncta on regions of the endoplasmic reticulum (ER) during interphase and at the cell plate during cell division. Without SABRE, cells accumulated ER aggregates and the ER abnormally buckled along the developing cell plate. Notably, callose deposition was delayed in ∆sabre, and in cells that failed to divide, abnormal callose accumulations formed at the cell plate. Our findings revealed a surprising and fundamental role for the ER in cell plate maturation.