Identification and Genetic Division of Fusarium graminearum and Fusarium asiaticum by Species‐Specific SCAR Markers
Identification and Genetic Division of Fusarium graminearum and Fusarium asiaticum by Species‐Specific SCAR Markers
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DOI：
10.1111/jph.12155
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发表时间：
2014-02
影响因子：
1.5
通讯作者：
Xu Zhang;Hongxiang Ma;Zhou Yongjin;Jincheng Xing;Jian-Hua Chen;Guihong Yu;Xiaobo Sun;W. Lei
中科院分区：
文献类型：
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作者：
Xu Zhang;Hongxiang Ma;Zhou Yongjin;Jincheng Xing;Jian-Hua Chen;Guihong Yu;Xiaobo Sun;W. Lei
赤霉病（Fusarium Head Blight，FHB）又称赤霉病（Scab），是一种危害小麦等谷类作物的毁灭性病害。和大麦（Hordeum vulgare L.）国际吧除了直接的产量损失外，最令人关注的是食物及生物受到霉菌毒素污染，对人畜健康构成威胁。最近的研究报告，系统发育的物种F。asiaticum（Fa）和F.禾谷镰刀菌（Fg）是我国小麦赤霉病的主要病原菌。为了利用种特异性和化学型特异性分子标记研究我国镰刀菌的种群结构，对我国镰刀菌代表菌株进行了SRAP标记筛选。asiaticum-NIV，F. asiaticum-3ADON和F. graminearum-15ADON来寻找物种或化学型特征的扩增产物。选择的扩增片段进行克隆和测序，使序列特征扩增区（SCAR）引物对可以开发，允许使用常规PCR的镰刀菌属物种的特异性检测。引物SCAR-Fa1和SCAR-Fg1仅在F.和F. graminearum分离物。利用这些种特异性引物对F. asiaticum和F.在江淮流域收集的禾本科菌株。结果表明，F.亚洲种是该麦区赤霉病的优势种。这是第一个报道，SRAP标记适用于镰刀菌菌株的物种鉴定。
Fusarium head blight (FHB), also called scab, is a devastating and insidious disease of cereals including wheat (Triticum spp.) and barley (Hordeum vulgare L.) worldwide. Apart from direct yield losses, the most serious concern about FHB is the contamination of the crop with mycotoxins, which pose a health risk to human and livestock. Recent research reported that phylogenetic species F. asiaticum (Fa) and F. graminearum (Fg) were the major causal agents of FHB from infected wheat heads in China. To investigate the population structure of Fusarium species in China by species-specific as well as the chemotype-specific markers, sequence-related amplified polymorphism (SRAP) markers were screened on representative isolates of F. asiaticum-NIV, F. asiaticum- 3ADON and F. graminearum-15ADON to find amplification products characteristic of either species or chemotypes. Selected amplified fragments were cloned and sequenced so that sequence-characterized amplified region (SCAR) primer pairs could be developed which permit specific detection of Fusarium species using conventional PCR. Primer pairs SCAR-Fa1 and SCAR-Fg1 were confirmed to be able to amplify specific products only in F. asiaticum and F. graminearum isolates, respectively. These species-specific primers were applied to determine genetic division of F. asiaticum and F. graminearum isolates collected in Yangtze–Huaihe valley. The results indicated that F. asiaticum was the predominant species causing FHB in this wheat production area. It is the first report that SRAP markers were adapted for species characterization in Fusarium isolates.