Molecular characterization of Cryptosporidium spp. in domestic pigeons (Columba livia domestica) in Guangdong Province, Southern China
Molecular characterization of Cryptosporidium spp. in domestic pigeons (Columba livia domestica) in Guangdong Province, Southern China
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DOI：
10.1007/s00436-015-4415-1
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发表时间：
2015-06-01
影响因子：
2
通讯作者：
Sun, Mingfei
中科院分区：
文献类型：
--
作者：
Li, Juan;Lin, Xuhui;Sun, Mingfei
为了解广东省家鸽隐孢子虫病的流行情况，评估家鸽隐孢子虫病的人畜共患传播负担，于2012年6月至2013年3月采集了广东省4个种鸽场的244份粪便样本。采用Sheather糖漂浮法纯化隐孢子虫卵囊，并对其小亚基核糖体RNA（SSU rRNA）基因进行序列测定。通过与GenBank中相应的隐孢子虫序列进行比较，并在MEGA5.2中使用邻接（NJ）进行系统发育分析，确定隐孢子虫物种。广东省家鸽隐孢子虫感染率为0.82%（2/244）。在惠州和潮州养殖场分别鉴定出2种隐孢子虫，即贝氏隐孢子虫和隐孢子虫。这些结果证实了C.本研究首次在国内家鸽中发现了猪隐翅虫感染，为进一步研究鸽对人类的公共卫生风险提供了基础资料。
To investigate the prevalence and assess the zoonotic transmission burden of Cryptosporidium species in domestic pigeons in Guangdong Province, Southern China, 244 fecal samples were collected from four pigeon breeding farms between June 2012 and March 2013. Cryptosporidium oocysts were purified by Sheather's sugar flotation technique and characterized by DNA sequencing of small subunit ribosomal RNA (SSU rRNA) gene. Cryptosporidium species were determined by comparison of sequences with corresponding Cryptosporidium sequences in GenBank and phylogenetic analysis using neighbor-joining (NJ) in MEGA5.2. The overall prevalence of Cryptosporidium infection in domestic pigeons in Guangdong Province was 0.82 % (2/244). Two Cryptosporidium species, namely Cryptosporidium baileyi and Cryptosporidium meleagridis, were identified in Huizhou and Chaozhou farm, respectively. These findings confirmed the existence of C. meleagridis infection in domestic pigeons in China for the first time and provided base-line information for further studies to evaluate the public health risk from pigeon to human.