Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.
Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.
复制标题
通过 CsCl 平衡梯度离心分离的人高密度脂蛋白亚组分中胆固醇和载脂蛋白 A-I 和 A-II 的分布：具有不同 A-I/A-II 摩尔比的 HDL 亚群的证据。
作者：
M. Cheung;J. Albers
本实验的目的是表征高密度脂蛋白（HDL）作为水合密度的函数。HDL的基础上，通过CsCl密度梯度离心的全血清或从三名男子和三名妇女分离的d 1.063 - 1.25 g/ml HDL馏分的水合密度细分。载脂蛋白A-I和A-II的放射免疫扩散定量表明，A-I/A-II的比值随脂蛋白水合密度而变化。在d 1.106和1.150 g/ml之间，HDL脂蛋白条带的A-I/A-II摩尔比几乎恒定在2.2 +/-0.2。在1.151 - 1.25 g/ml的密度范围内，A-I/A-II比值随密度增加而增加。另一方面，在1.077和1.105之间的密度范围内，A-I/A-II比率随着密度降低而增加，范围从d 1.093 - 1.105 g/ml级分的2.8 +/-0.5到d 1.077 - 1.082 g/ml级分的5.6 +/-1.3。d 1.063 - 1.076 g/ml组分和d 1.077 - 1.082 g/ml组分的A-I/A-II比值相当。血清和d 1.063 - 1.25 g/ml HDL组分表现出相似的趋势。在所有12个检查的样品（6个血清和6个HDL）中，胆固醇/（A-I + A-II）比值随着密度的增加而降低。密度梯度组分的梯度凝胶电泳显示，随着密度从1.063 g/ml增加到1.200 g/ml，表观分子量从3.9 x 10（5）降低到1.1 x 10（5）。当从男性或女性中分离时，具有相同水合密度的HDL亚组分具有可比的分子量和A-I/A-II和胆固醇/（A-I + A-II）比率。HDL包含A-I/A-II摩尔比不同的亚群。张，M. C.的方法，和J. J.阿尔伯斯。CsCl平衡梯度离心分离的人高密度脂蛋白亚组分中胆固醇和载脂蛋白A-I和A-II的分布：具有不同A-I/A-II摩尔比的HDL亚群的证据
The purpose of this experiment was to characterize the high density lipoproteins (HDL) as a function of hydrated density. HDL was subfractionated on the basis of hydrated density by CsCl density gradient centrifugation of whole serum or the d 1.063-1.25 g/ml HDL fraction isolated from three men and three women. Apolipoprotein A-I and A-II quantitation by radial immunodiffusion showed that the A-I/A-II ratio varied with the lipoprotein hydrated density. The A-I/A-II molar ratio of HDL lipoproteins banding between d 1.106 and 1.150 g/ml was nearly constant at 2.2 +/- 0.2. In the density range 1.151-1.25 g/ml the A-I/A-II ratio increased as the density increased. On the other hand, in the density range between 1.077 and 1.105 the A-I/A-II ratio increased as the density decreased, ranging from 2.8 +/- 0.5 for the d 1.093-1.105 g/ml fraction to 5.6 +/- 1.3 for the d 1.077-1.082 g/ml fraction. The d 1.063-1.076 g/ml fraction and the d 1.077-1.082 g/ml fractions had comparable A-I/A-II ratios. Serum and the d 1.063-1.25 g/ml HDL fraction exhibited similar trends. The cholesterol/(A-I + A-II) ratio decreased as the density increased in all 12 samples (six serum and six HDL) examined. Gradient gel electrophoresis of the density gradient fractions showed that as the density increased from 1.063 to 1.200 g/ml the apparent molecular weight decreased from 3.9 x 10(5) to 1.1 x 10(5). HDL subfractions with the same hydrated densities had comparable molecular weights and A-I/A-II and cholesterol/(A-I + A-II) ratios when isolated from men or women. HDL contains subpopulations that differ in the A-I/A-II molar ratio.-Cheung, M. C., and J. J. Albers. Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.