The role of mucus in antibody-mediated rapid expulsion of Trichinella spiralis in suckling rats.
The role of mucus in antibody-mediated rapid expulsion of Trichinella spiralis in suckling rats.
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发表时间：
1990-05
期刊：
影响因子：
6.4
通讯作者：
M. S. Carlisle;D. McGregor;J. Appleton
中科院分区：
文献类型：
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作者：
M. S. Carlisle;D. McGregor;J. Appleton
被旋毛虫寄生的哺乳母鼠的幼鼠表现出快速排出，这是一种保护性反应，与肠道粘液中感染性肌幼虫的捕获有关。免疫荧光研究表明，抗体结合到表面的诱捕幼虫。粘液结合和快速排出发生在正常幼仔给药幼虫涂有抗体制备的感染大鼠血清。随后的实验表明，诱捕的幼虫在盐水中体外孵育2小时后从粘液中逃脱。逃逸与表面结合的抗体的损失，表明粘液截留是可逆的，并依赖于抗体涂层。最后，当保护性抗体注射后1，2或6小时，幼虫给药的幼崽，寄生虫被迫离开他们的上皮龛，并成为粘液包膜。上述结果提示T.旋毛虫幼虫依赖于抗体对幼虫的包被，但这种诱捕是可逆的，其本身并不是快速驱逐的关键事件。快速排出的主要机制似乎是抗体介导的抑制寄生虫在上皮中维持自身所需的过程。
Rat pups suckling dams parasitized by Trichinella spiralis express rapid expulsion, a protective response that is associated with the entrapment of infectious muscle larvae in intestinal mucus. Immunofluorescent studies revealed that antibodies were bound to the surfaces of the entrapped larvae. Mucus binding and rapid expulsion occurred in normal pups dosed with larvae coated with antibodies prepared from infected rat serum. Subsequent experiments revealed that entrapped larvae escaped from mucus after 2 hr in vitro incubation in saline. Escape correlated with the loss of the surface-bound antibodies, suggesting that mucus entrapment was reversible and dependent on antibody coating. Finally, when protective antibodies were injected 1, 2 or 6 hr after larvae were administered to pups, the parasites were forced to leave their epithelial niche and became enveloped in mucus. The above findings suggest that mucus trapping of T. spiralis larvae is dependent upon the coating of larvae by antibody, but that trapping is reversible, and is not in itself the pivotal event in rapid expulsion. The primary mechanism of rapid expulsion appears to be antibody-mediated inhibition of processes required for the parasite to maintain itself in the epithelium.