Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis
Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis
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DOI：
10.1128/jb.186.8.2487-2491.2004
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发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Andersen, P
中科院分区：
文献类型：
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作者：
Okkels, LM;Andersen, P
在本研究中，我们证明，与编码ESAT-6和CFP-10的基因类似，来自ESAT-6基因家族的基因rv 0287和rv 0288是共转录的。使用蛋白质印迹和蛋白质印迹覆盖方法，我们证明了ESAT-6和CFP-10，以及蛋白质对Rv 0288/Rv 0287，以高度特异性的方式成对相互作用。最值得注意的是，ESAT-6蛋白直接与Rv 3873相互作用，Rv 3873是ESAT-6分泌途径的可能细胞包膜组分。
In the present study, we demonstrate that, in analogy with the genes encoding ESAT-6 and CFP-10, the genes rv0287 and rv0288 from the ESAT-6 gene family are cotranscribed. Using Western-Western blotting and protein-print overlay methodologies, we demonstrate that ESAT-6 and CFP-10, as well as the protein pair Rv0288/Rv0287, interact pairwise in a highly specific way. Most notably, the ESAT-6 proteins interact directly with Rv3873, a possible cell envelope component of the ESAT-6 secretion pathway.