A combination of tea (Camellia senensis) catechins is required for optimal inhibition of induced CYP1A expression by green tea extract.
A combination of tea (Camellia senensis) catechins is required for optimal inhibition of induced CYP1A expression by green tea extract.
复制标题
需要茶 (Camellia senensis) 儿茶素组合才能最佳地抑制绿茶提取物诱导的 CYP1A 表达。
DOI：
10.1021/jf030181z
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发表时间：
2003
影响因子：
6.1
通讯作者：
Quattrochi,LindaC
中科院分区：
文献类型：
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作者：
Williams,SusanneN;Pickwell,GeorgeV;Quattrochi,LindaC
先前已经证明，商业绿色茶提取物Polyphenon 100（P100）和较小程度的（-）-表没食子儿茶素-3-没食子酸酯（EGCG）部分拮抗2，3，7，8-四氯二苯并-p-二恶英（TCDD）诱导的人CYP 1A1转录（威廉姆斯，S. N.的; Shih，H.; Guenette，D. K.的; Brackney，W.; Denison，M. S.的; Pickwell，G.五、夸特罗奇湖C. Chem. -Biol. Interact.2000，128，211 - 229）。这里，将P100与四种主要茶儿茶素的重构混合物（称为P100R）进行比较，以确定抑制是否是由于提取物中的额外多酚或来自茶儿茶素之间的协同相互作用。结果发现，TCDD和P100或P100R共同处理细胞抑制TCDD诱导的CYP 1A启动子驱动的荧光素酶报告活性（HepG2细胞）和CYP 1A表达（HepG2和原代人肝细胞），类似。这些结果表明，P100对人CYP 1A表达的调节可以完全归因于四种茶儿茶素的组合。这些发现可能是重要的评价未来的化学预防策略使用绿色茶和单一的儿茶素agents. Keywords：绿色茶;山茶;多酚;儿茶素;细胞色素P450; CYP1A;人肝细胞
It was previously demonstrated that the commercial green tea extract Polyphenon 100 (P100), and to a lesser extent (−)-epigallocatechin-3-gallate (EGCG), partially antagonizes 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced transcription of humanCYP1A1(Williams, S. N.; Shih, H.; Guenette, D. K.; Brackney, W.; Denison, M. S.; Pickwell, G. V.; Quattrochi, L. C.Chem.−Biol. Interact.2000,128, 211−229). Here, P100 is compared to a reconstituted mixture of the four major tea catechins (referred to as P100R) to determine whether inhibition was due to additional polyphenols in the extract or from synergistic interactions among the tea catechins. It was found that cotreatment of cells with TCDD and either P100 or P100R inhibited TCDD-inducedCYP1Apromoter-driven luciferase reporter activity (HepG2 cells) andCYP1Aexpression (HepG2 and primary human hepatocytes), similarly. These results indicate that modulation of humanCYP1Aexpression by P100 can be attributed entirely to the combination of the four tea catechins. These findings may be important in the evaluation of future chemoprevention strategies using green tea and single catechin agents.Keywords: Green tea;Camellia senensis; polyphenols; catechins; cytochrome P450;CYP1A; human hepatocytes