Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates.
Understanding Peptide Oligomeric State in Langmuir Monolayers of Amphiphilic 3-Helix Bundle-Forming Peptide-PEG Conjugates.
复制标题
了解两亲性 3 螺旋束形成肽-PEG 缀合物的朗缪尔单层中的肽寡聚状态。
DOI：
10.1021/acs.biomac.6b01356
复制
发表时间：
2016
影响因子：
6.2
通讯作者：
Xu,Ting
中科院分区：
文献类型：
--
作者：
Lund,Reidar;Ang,JooChuan;Shu,JessicaY;Xu,Ting
盘状多肽-聚合物偶联物是一类新兴的生物材料。基本了解这些混合构建块的盘绕低聚状态和组装过程对于控制它们组装成明确定义的结构是必要的。在这里，我们用X射线反射率(XR)和掠入射X射线衍射(GIXD)研究了多肽结构和聚乙二醇化对两亲性螺旋卷曲多肽-聚合物共轭朗缪尔单分子膜自组装过程和低聚状态的影响。我们的结果表明，基于3-螺旋束形成肽的聚乙二醇化两亲物的低聚状态与表面压力有关，在中等表面压力下形成二聚体和三聚体的混合物，但随着表面压力的增加完全转变为三聚体。此外，在高表面压力下，3-螺旋多肽-聚乙二醇共轭两亲分子的螺旋间距不受扰动。目前的研究为多肽-聚合物杂化偶联物的自组装过程提供了有价值的见解，并为开发具有可控多价态配体呈现的生物材料提供了指导。
Coiled-coil peptide–polymer conjugates are an emerging class of biomaterials. Fundamental understanding of the coiled-coil oligomeric state and assembly process of these hybrid building blocks is necessary to exert control over their assembly into well-defined structures. Here, we studied the effect of peptide structure and PEGylation on the self-assembly process and oligomeric state of a Langmuir monolayer of amphiphilic coiled-coil peptide–polymer conjugates using X-ray reflectivity (XR) and grazing-incidence X-ray diffraction (GIXD). Our results show that the oligomeric state of PEGylated amphiphiles based on 3-helix bundle-forming peptide is surface pressure dependent, a mixture of dimers and trimers was formed at intermediate surface pressure but transitions into trimers completely upon increasing surface pressure. Moreover, the interhelical distance within the coiled-coil bundle of 3-helix peptide-PEG conjugate amphiphiles was not perturbed under high surface pressure. Present studies provide valuable insights into the self-assembly process of hybrid peptide–polymer conjugates and guidance to develop biomaterials with controlled multivalency of ligand presentation.