Effect of molasses on the production and activity of dye-decolorizing peroxidase from Geotrichum candidum Dec1
Effect of molasses on the production and activity of dye-decolorizing peroxidase from Geotrichum candidum Dec1
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DOI：
10.1016/s1389-1723(00)80054-7
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发表时间：
2000-06-01
影响因子：
2.8
通讯作者：
Shoda, M
中科院分区：
文献类型：
--
作者：
Lee, TH;Aoki, H;Shoda, M
研究了以糖蜜为碳源，培养铁皮地霉（Geotrichum candidum Dec1）生产染料脱色过氧化物酶（DyP）的方法。当糖蜜浓度大于10 g.l（-1）时，发现培养液对染料活性蓝5的脱色活性增加，主要是因为产生的酶量增加。而在20 g.l（-1）浓度下，糖蜜对DyP活性有完全抑制作用，说明糖蜜对培养液脱色活性有抑制作用。当培养液稀释25倍时，其染料脱色活性是未稀释培养液的7倍。凝胶层析分离的糖蜜部分（300-400 ml和400-500 ml部分）完全抑制纯化的DyP。在这些结果的基础上，我们提出了一种方案来控制糖蜜对染料脱色过程的积极和消极影响。
The production of dye-decolorizing peroxidase (DyP) was investigated by cultivating Geotrichum candidum Dec1 using molasses as a carbon source. Molasses at concentrations greater than 10 g.l(-1) was found to increase the decolorization activity of the culture broth toward dye, reactive blue 5 mainly because the amount of enzyme produced was enhanced. However, complete inhibition of DyP activity by molasses was observed at the concentration of 20 g.l(-1), indicating that the inhibitory effect of molasses on the culture broth activity to decolorize the dye was involved. When the culture broth was diluted 25 times, the dye-decolorizing activity was 7 times as much as that of non-diluted culture broth. The molasses fractions separated by gel chromatography (300-400 ml and 400-500 ml fractions) completely inhibited the purified DyP. On the basis of these results, we propose a scheme to control both positive and negative effects of molasses on the dye decolorization process.