Locomotor activity in males of Aedes aegypti can shift in response to females' presence
Locomotor activity in males of Aedes aegypti can shift in response to females' presence
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DOI：
10.1186/s13071-018-2635-9
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发表时间：
2018-04-18
影响因子：
3.2
通讯作者：
Bruno, Rafaela Vieira
中科院分区：
文献类型：
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作者：
Araripe, Luciana Ordunha;Assuncao Bezerra, Jessica Rodrigues;Bruno, Rafaela Vieira
背景：对蚊子媒介的生理和行为特征的研究，与控制媒介传播疾病的替代方法的主张相关。尽管如此，大多数研究都集中在女性的特征上，包括寻求宿主的行为，疾病传播的生理学以及用于产卵的现场选择。但是，了解导致男性生殖成功的因素至关重要，因为它可以帮助制定限制人口增长的新策略。男性对交配的行为在物种之间差异很大，男性与女性之间的交流是确保成功相遇的第一个方面。在这里，我们使用了一个自动监测系统来研究埃及埃及男性运动活动的特征，以响应女性在适应的约束管中的存在。我们提出了一种新方法来量化男性对女性存在的反应，可以潜在地测试一个男性在识别女性交配时成功的指标。染色：运动活性在每日周期中由内源性时钟调节，并由外部因素同步，例如光和温度。我们的结果表明，先前描述的惊吓响应对光，当灯打开时，立即显示为陡峭的早晨活动峰。白天的活动下降，在傍晚之前开始再次上升，男性比女性早1.5 h。最有趣的是，雄性活动显示出双峰，而当男性单独且在限制管中存在女性时，第二个峰非常微妙，并且相对明显。通过此处建议的峰匹配指数（PMI）测量的活动峰的转换在有和没有女性的男性之间显着差异。结论：这里使用的适应性监测系统使我们能够按照活动峰值的程度量化男性对附近女性对附近女性的反应。在这个方向上，我们创建了峰匹配指数（PMI），我们预期的一个新参数可以解释为雄性倾向以应对女性的存在，并进一步测试是寻找女性交配的潜力的指标。
Background: The study of physiological and behavioral traits of mosquito vectors has been of growing relevance for the proposition of alternative methods for controlling vector-borne diseases. Despite this, most studies focus on the female's traits, including the behavior of host seeking, the physiology of disease transmission and the site-choice for oviposition. However, understanding the factors that lead to males' reproductive success is of utmost importance, since it can help building new strategies for constraining population growth. Male behavior towards mating varies widely among species and the communication between males and females is the first aspect securing a successful encounter. Here we used an automated monitoring system to study the profile of locomotor activity of Aedes aegypti males in response to female's presence in an adapted confinement tube. We propose a new method to quantify male response to the presence of females, which can be potentially tested as an indicator of the success of one male in recognizing a female for mating.Results: Locomotor activity varies in daily cycles regulated by an endogenous clock and synchronized by external factors, such as light and temperature. Our results show the previously described startle response to light, which is displayed as a steep morning activity peak immediately when lights are on. Activity drops during the day and begins to rise again right before evening, happening about 1.5 h earlier in males than in females. Most interestingly, males' activity shows a double peak, and the second peak is very subtle when males are alone and relatively more pronounced when females are present in the confinement tubes. The switch in the peak of activity, measured by the herein suggested Peak Matching Index (PMI), was significantly different between males with and without females.Conclusions: The adapted monitoring system used here allowed us to quantify the response of individual males to nearby females in terms of the extent of the activity peak displacement. In this direction, we created the peak matching index (PMI), a new parameter that we anticipate could be interpreted as the inclination of males to respond to females' presence, and further tested as an indicator of the potential for finding females for mating.