Nucleotide sequence diversity of the 5S rDNA spacer in the simple blade kelp genera Laminaria, Cymathaere and Kjellmaniella (Laminariales, Phaeophyceae) from northern Japan
Nucleotide sequence diversity of the 5S rDNA spacer in the simple blade kelp genera Laminaria, Cymathaere and Kjellmaniella (Laminariales, Phaeophyceae) from northern Japan
复制标题
DOI：
10.1111/j.1440-1835.2006.00434.x
复制
发表时间：
2006-12
影响因子：
1.5
通讯作者：
N. Yotsukura;T. Kawai;S. Kawashima;Hiroki Ebata;T. Ichimura
中科院分区：
文献类型：
--
作者：
N. Yotsukura;T. Kawai;S. Kawashima;Hiroki Ebata;T. Ichimura
在日本北部分布的几乎所有海带动物、cymathaeean和kjellmaniellan物种中都证实了5S核糖体RNA基因（rDNA）串联重复序列。对31个位点79个样本的串联重复5S rDNA间隔区核苷酸序列进行了研究。对29个不同序列的系统发育分析表明：(1)海带类群，包括：海带、拟海带、海带（Miyabe）、海带（Miyabe）、海带（yendoana Miyabe）、日本海带（Cymathaere japonica Miyabe et Nagai）、gyrata kjellmanella Miyabe和kjellmanella crassifolia Miyabe；(2)日本海带类群，包括日本海带、宫贝海带、宫贝海带、宫贝海带、宫贝海带、长足海带、海带和宫贝长足海带。后一类群又分为两个亚群（2a），包括长叶橐吾（L. angustata）和长叶橐吾（L. longissima），以及长叶橐吾（L. religiosa）、长叶橐吾（L. ochotensis）、长叶橐吾（L. diabolica）和长叶橐吾（L. longpedalis）。这三组的成员在叶片表面的装饰（凸起，旋转和褶皱）的外观上彼此不同。这些在组（2a）中不存在，仅在组（2b）的早期阶段存在，并且在组(1)的整个生命周期中都存在。组内样本间的遗传距离极小（2a）。结合之前的交叉研究以及洋流和分布的数据，这些发现表明基因流动发生在组内（2b）。
Tandem repeats of the 5S ribosomal RNA gene (rDNA) were confirmed for almost all laminarian, cymathaerean and kjellmaniellan species distributed in northern Japan. The nucleotide sequence of the spacer region between tandemly repeated 5S rDNA was investigated for 79 samples from 31 sites. Phylogenetic analysis of the 29 different sequences detected revealed two lineages: (1) Laminaria coriacea group, including Laminaria coriacea Miyabe, Laminaria cichorioides Miyabe, Laminaria sachalinensis (Miyabe) Miyabe, Laminaria yendoana Miyabe, Cymathaere japonica Miyabe et Nagai, Kjellmaniella gyrata (Kjellman) Miyabe and Kjellmaniella crassifolia Miyabe; (2) Laminaria japonica group including Laminaria japonica Areschoug, Laminaria religiosa Miyabe, Laminaria ochotensis Miyabe, Laminaria diabolica Miyabe, Laminaria longipedalis Okamura, Laminaria angustata Kjellman and Laminaria longissima Miyabe. In addition, the latter group was divided into two: subgroup (2a) including L. angustata and L. longissima and subgroup (2b) including L. japonica, L. religiosa, L. ochotensis, L. diabolica and L. longipedalis. Members of the three groups differ from each other in the appearance of ornaments (bullation, gyration and folds) on the surface of the blade. These are absent in group (2a), only present in the early stages of the lifespan of group (2b), and present for the duration of the lifespan in group (1). Genetic distances among samples were extremely small within group (2a). Together with previous crossing studies and data on ocean currents and distribution, these findings suggest that gene flow occurs within group (2b).